Dietary Lipid During Late-Pregnancy and Early-Lactation to Manipulate Metabolic and Inflammatory Gene Network Expression in Dairy Cattle Liver with a Focus on PPARs.
Dietary Lipid During Late-Pregnancy and Early-Lactation to Manipulate Metabolic and Inflammatory Gene Network Expression in Dairy Cattle Liver with a Focus on PPARs.
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DOI：
10.4137/grsb.s12005
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Loor JJ
中科院分区：
文献类型：
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作者：
Akbar H;Schmitt E;Ballou MA;Corrêa MN;Depeters EJ;Loor JJ
多不饱和脂肪酸（PUFA）长链脂肪酸（LCFA）比饱和LCFA更能引起单胃动物的分子和组织功能变化。从相对于分娩的第-21天到第10天，奶牛没有补充LCFA（对照），饱和LCFA（SFAT;主要是16：0和18：0）或鱼油（FISH;高PUFA）。在第-14天和第10天，通过定量RT-PCR测量肝脏组织中的27个基因。在第-14天，SFAT组的核受体辅激活因子（CARM 1、MED 1）、LCFA代谢（ACSL 1、SCD、ACOX 1）和炎症（IL 6、TBK 1、IKBKE）基因的表达低于对照组。然而，在-14天和10天时，FISH的SCD表达明显低于对照或SFAT。FISH导致第10天LCFA代谢（CD 36、PLIN 2、ACSL 1、ACOX 1）、细胞内能量（UCP 2、STK 11、PRKAA 1）、从头胆固醇合成（SREBF 2）、炎症（IL 6、TBK 1、IKBKE）和核受体信号传导基因（PPARD、MED 1、NRIP 1）的表达进一步降低。未观察到PPARA和RXRA的表达变化。在饲喂SFAT的奶牛中，第10天DGAT 2、PLIN 2、ACSL 1和ACOX 1的增加与第-14天相比，表明β-氧化和脂滴（LD）形成均上调。然而，肝脏三酰甘油浓度是相似的治疗。在第10天，肝细胞因子FGF 21和致癌基因PC和PCK 1显著增加，而对照组仅在-14天。在补充的水平上，在饲喂饱和脂肪的奶牛分娩后代谢基因谱的变化表明，脂肪酸的摄取和细胞内处理的能力更大，而不需要过多的LD储存。
Polyunsaturated (PUFA) long-chain fatty acids (LCFAs) are more potent in eliciting molecular and tissue functional changes in monogastrics than saturated LCFA. From −21 through 10 days relative to parturition dairy cows were fed no supplemental LCFA (control), saturated LCFA (SFAT; mainly 16:0 and 18:0), or fish oil (FISH; high-PUFA). Twenty-seven genes were measured via quantitative RT-PCR in liver tissue on day −14 and day 10. Expression of nuclear receptor co-activators (CARM1, MED1), LCFA metabolism (ACSL1, SCD, ACOX1), and inflammation (IL6, TBK1, IKBKE) genes was lower with SFAT than control on day −14. Expression of SCD, however, was markedly lower with FISH than control or SFAT on both −14 and 10 days. FISH led to further decreases in expression on day 10 of LCFA metabolism (CD36, PLIN2, ACSL1, ACOX1), intracellular energy (UCP2, STK11, PRKAA1), de novo cholesterol synthesis (SREBF2), inflammation (IL6, TBK1, IKBKE), and nuclear receptor signaling genes (PPARD, MED1, NRIP1). No change in expression was observed for PPARA and RXRA. The increase of DGAT2, PLIN2, ACSL1, and ACOX1 on day 10 versus −14 in cows fed SFAT suggested upregulation of both beta-oxidation and lipid droplet (LD) formation. However, liver triacylglycerol concentration was similar among treatments. The hepatokine FGF21 and the gluconeogenic genes PC and PCK1 increased markedly on day 10 versus −14 only in controls. At the levels supplemented, the change in the profile of metabolic genes after parturition in cows fed saturated fat suggested a greater capacity for uptake of fatty acids and intracellular handling without excessive storage of LD.