Di fferential gene expression in foxtail millet during interaction with the smut fungus Ustilago crameri
Di fferential gene expression in foxtail millet during interaction with the smut fungus Ustilago crameri
复制标题
谷子与黑粉菌黑粉菌相互作用过程中的差异基因表达
DOI：
10.1016/j.pmpp.2020.101459
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发表时间：
2020-04-01
影响因子：
2.7
通讯作者：
Yi, Huilan
中科院分区：
文献类型：
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作者：
Hao, Lihong;Liu, Jing;Yi, Huilan
谷子黑粉病是由担子菌Ustilago Crameri引起的一种严重危害谷子生产的病害。然而，对谷子对克雷梅氏杆菌侵染的分子反应知之甚少。本文利用RNA-Seq技术对抗病品种冀谷20和感病品种长农35的转录调控进行了研究。在冀谷20和长农35中分别鉴定出1712个差异表达基因和1166个差异表达基因。结果表明，病程相关(PR)基因SiBGL和SiCHI在冀谷20号和长农35号上对克雷梅氏菌的接种均有阳性反应。抗性(R)基因SiRPM1及其下游信号分子SiSGT1和SiHSP也在黑穗病侵染后显著上调。此外，我们还揭示了SiCDPK-SiRbohF介导的ROS的产生可能对谷子对克氏原虫的防御起到了积极的作用。此外，我们还发现，在黑穗病侵染过程中，苯丙烷途径被激活。综上所述，我们的结果表明，SiBGL、SiCHI、SiRPM1、SiSGT1、SiHSP、SiCDPK、SiRbohF和SiPAL可能是谷子抗黑穗病的候选基因。
Foxtail millet smut disease, which is caused by the basidiomycetous fungus Ustilago crameri , has become a severe detriment to millet production. However, little is known about the molecular responses of foxtail millet to infection with U. crameri . In this article, we performed RNA-Seq to investigate transcriptional modulation in the resistant cultivar Jigu20 and the susceptible cultivar Changnong35. In total, 1712 and 1166 di fferentially ex- pressed genes (DEGs) were identi fied in Jigu20 and Changnong35, respectively. Our results showed that the pathogenesis -related (PR) genes SiBGL and SiCHI responded positively to inoculation with U. crameri in both Jigu20 and Changnong35. The resistance (R) gene SiRPM1 and its downstream signaling molecules SiSGT1 and SiHSP were also dramatically upregulated upon smut infection in Jigu20. Moreover, we revealed that SiCDPK- SiRbohF -mediated reactive oxygen species (ROS) production might positively contribute to foxtail millet defense against U. crameri . Furthermore, we found that the phenylpropanoid pathway was activated upon smut infection. Taken together, our results suggested that SiBGL , SiCHI , SiRPM1 , SiSGT1 , SiHSP , SiCDPK , SiRbohF and SiPAL could act as potential candidates in foxtail millet to achieve resistance against smut.