BIOSYNTHESIS OF ASPARAGINE-LINKED OLIGOSACCHARIDES IN SACCHAROMYCES-CEREVISIAE - THE ALG2 MUTATION
BIOSYNTHESIS OF ASPARAGINE-LINKED OLIGOSACCHARIDES IN SACCHAROMYCES-CEREVISIAE - THE ALG2 MUTATION
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DOI：
10.1093/glycob/3.4.357
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发表时间：
1993-08-01
期刊：
影响因子：
4.3
通讯作者：
ROBBINS, P
中科院分区：
文献类型：
--
作者：
JACKSON, BJ;KUKURUZINSKA, MA;ROBBINS, P
在酵母酿酒酵母中，alg 2突变导致温度敏感性生长和脂质连接的寡糖Man 2GlcNAc 2-PP-Dol的异常积累（杰克逊et al.，生物化学与生物物理学，272，203-209，1989; Huffaker和Robbins，Proc. Natl. Acad. Sci. USA，80，7466-7470，1983）。从基于多拷贝质粒YEp 24和着丝粒质粒YCp 50的文库中克隆具有ALG 2的功能和基因组位置的基因。用含有ALG 2的质粒转化的Alg 2突变体恢复了在以前不允许的温度下正常生长和合成脂质连接低聚糖的能力。ALG 2在单倍体和二倍体酵母中是生存所必需的。ALG 2基因被转录成大小为1.7kb的单个mRNA。在rpb 1 -1突变体中RNA聚合酶II热灭活后评估的ALG 2 mRNA的稳定性（Herrick et al.，摩尔Cell.生物学，10，2269-2284，1990）非常低，具有在外链形成期间伸长的核心的3个分支的t1/2（Mrsu等人，Proc. Natl. Acad. Sci. USA，87，3368-3372，1990; Jiangsu等人，Proc. Natl. Acad. Sci. USA，83，3081-3085，1986）。
In the yeast Saccharomyces cerevisiae, the alg2 mutation causes temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol (Jackson et al., Arch. Biochem. Biophys., 272, 203-209, 1989; Huffaker and Robbins, Proc. Natl. Acad. Sci. USA, 80, 7466-7470, 1983). A gene having the function and genomic location of ALG2 was cloned from libraries based on the multicopy plasmid YEp24 and on the centromere plasmid YCp50. Alg2 mutants transformed with plasmids containing ALG2 regained the capacity to grow and to synthesize lipid-linked oligosaccharides normally at the previously non-permissive temperature. ALG2 was essential for viability in haploid and diploid yeast. The ALG2 gene was transcribed into a single mRNA of 1.7 kb in size. The stability of ALG2 mRNA, assessed after thermal inactivation of RNA polymerase II in an rpb1-1 mutant (Herrick et al., Mol. Cell. Biol., 10, 2269-2284, 1990) was very low, with a t1/2 of 3 branch of the core that is elongated during outer chain formation (Ballou et al., Proc. Natl. Acad. Sci. USA, 87, 3368-3372, 1990; Ballou et al., Proc. Natl. Acad. Sci. USA, 83, 3081-3085, 1986).