Human salivary gland stem/progenitor cells remain dormant even after irradiation
Human salivary gland stem/progenitor cells remain dormant even after irradiation
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DOI：
10.3892/ijmm_00000240
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发表时间：
2009-09-01
影响因子：
5.4
通讯作者：
Tohnai, Iwai
中科院分区：
文献类型：
--
作者：
Tatsuishi, Yuki;Hirota, Makoto;Tohnai, Iwai
在接受头颈部放射治疗的大多数患者中，唾液腺功能会逐渐丧失。目前尚不清楚成人唾液腺组织中是否含有干细胞/祖细胞。在这项研究中，我们使用集落试验来确定照射后成人颌下腺中是否存在干细胞/祖细胞。我们开发了一种新的培养体系，用低密度培养照射和未照射的成人颌下腺细胞，在添加血清的基础上用无血清培养促进单细胞克隆形成。所有样本的细胞，除了从接受最高辐射剂量的最年长患者获得的细胞外，通过逆转录-聚合酶链式反应(RT-PCR)和免疫染色表达腺泡、导管和肌上皮细胞系标记。在无血清培养条件下，这些菌落的传代培养显示出高度的多能性。这些结果首次描述在唾液腺中存在具有自我更新、高增殖和多向分化活性的唾液腺干/祖细胞，即使在照射后也是如此。细胞的存活取决于辐射剂量和细胞老化。
Progressive loss of salivary gland function occurs in most patients undergoing head and neck radiotherapy. It is unclear whether adult salivary gland tissue contains stem/progenitor cells. In this study, we used a colony assay to clarify the presence of stem/progenitor cells in adult submandibular glands after irradiation. We developed a novel culture system that promotes single-cell colony formation with low density culture of irradiated and non-irradiated adult human submandibular gland cells using serum-free medium following serum-supplemented medium. The cells from all samples, except those obtained from the oldest patient who received the highest radiation dose, expressed acinar, ductal, and myoepithelial cell-lineage markers with reverse transcription-polymerase chain reaction (RT-PCR) and immunostaining. A sub-culture of these colonies with serum-free medium showed high multipotency. These results are the first description of presence of salivary gland stem/progenitor cells with self-renewal, high proliferation and multipotent differentiation activity in salivary glands, even after irradiation. The survival of the cells depends on radiation dose and cell aging.