Rabbit tendon cells produce MMP-3 in response to fluid flow without significant calcium transients
Rabbit tendon cells produce MMP-3 in response to fluid flow without significant calcium transients
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DOI：
10.1016/s0021-9290(01)00217-2
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发表时间：
2002-03-01
影响因子：
2.4
通讯作者：
Banes, AJ
中科院分区：
文献类型：
--
作者：
Archambault, JM;Elfervig-Wall, MK;Banes, AJ
在运动过程中施加于肌腱的力会导致细胞变形以及流体运动。本研究的目的是验证兔肌腱成纤维细胞检测并响应流体诱导的剪切应力的假设。从兔跟腱副腱中分离细胞，在特制的多滑动流动装置中以1 dyn/cm(2)的速度流动6 h。液体流动的应用导致胶原酶-1 （MMP-1）、基质溶素-1 （MMP-3）、环氧化酶II （COX-2）和白细胞介素-1 β (il -1 β)基因的表达增加。介质中proMMP-3的释放与流体剪切应力水平呈剂量响应关系。然而，并非所有细胞都与流动方向一致。在其他实验中，同样的细胞用钙活性染料FURA-2 AM孵育，然后在平行板流室中进行层流流动。当暴露于高达25 dyn/CM2的流体剪切应力水平时，细胞内钙浓度没有显著增加。这些结果表明，兔肌腱细胞的基因表达对流体流动敏感，但信号转导不依赖于细胞内钙瞬变。MMP-1、MMP-3和COX-2基因的上调表明，流体流动可能是肌腱重塑或损伤的重要机械刺激。(C) 2002 Elsevier Science Ltd.版权所有。
Forces applied to tendon during movement cause cellular deformation, as well as fluid movement. The goal of this study was to test the hypothesis that rabbit tendon fibroblasts detect and respond to fluid-induced shear stress. Cells were isolated from the paratenon of the rabbit Achilles tendon and then subjected to fluid flow at 1 dyn/cm(2) for 6 h in a specially designed multi-slide flow device. The application of fluid flow led to an increased expression of the collagenase-1 (MMP-1), stromelysin-1 (MMP-3), cyclooxygenase II (COX-2) and interleukin-1beta (IL-1beta) genes. The release of proMMP-3 into the medium exhibited a dose-response with the level of fluid shear stress. However, not all cells aligned in the direction of flow. In other experiments, the same cells were incubated with the calcium-reactive dye FURA-2 AM, then subjected to laminar fluid flow in a parallel plate flow chamber. The cells did not significantly increase intracellular calcium concentration when exposed to fluid shear stress levels of up to 25 dyn/CM2. These results show that gene expression in rabbit tendon cells is sensitive to fluid flow, but that signal transduction is not dependent on intracellular calcium transients. The upregulation of the MMP-1, MMP-3 and COX-2 genes shows that fluid flow could be an important mechanical stimulus for tendon remodelling or injury. (C) 2002 Elsevier Science Ltd. All rights reserved.