Differences in circulating dendritic cell subtypes in cord blood and peripheral blood of healthy and allergic children
Differences in circulating dendritic cell subtypes in cord blood and peripheral blood of healthy and allergic children
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DOI：
10.1046/j.1365-2222.2003.01649.x
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发表时间：
2003-05-01
影响因子：
6.1
通讯作者：
Stevens, WJ
中科院分区：
文献类型：
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作者：
Hagendorens, MM;Ebo, DG;Stevens, WJ
不同类型的循环树突状细胞已被描述。树突状细胞影响幼稚T淋巴细胞向辅助T型1 （Th1）和Th2效应细胞的分化。目的评价过敏儿童和健康儿童外周血、过敏父母和非过敏父母新生儿脐带血循环DC亚型的数量。方法采用流式细胞术对全血循环树突状细胞进行CD3、CD14、CD16、CD19、CD20、CD56阴性、CD34阴性和hla - dr阳性细胞系鉴定。根据CD123和CD11c的表达，鉴定出不同的DC亚型。除了DC1 (CD11c(+) CD123（dim+）和DC2 (CD11c（-) CD123（高+））外，第三个DC群体被描述为具有低分化表型特征，即CD11c(-) CD123(dim+)，因此在这里定义为低分化DC （ldDC）。这些ldDC代表了脐带血中主要的DC人群，并呈现出年龄依赖性下降。在患有特应性皮炎的儿童中检测到最高水平的ldDC，而哮喘儿童的ldDC计数最低。此外，哮喘儿童吸入大剂量皮质类固醇治疗与ldDC数降低有关。与健康儿童相比，过敏儿童，尤其是哮喘儿童的循环DC2数量明显较低。在脐带血中，在过敏性疾病低风险和高风险的新生儿中没有检测到DC亚型的差异。结论这些结果表明，除了DC1和DC2外，还有第三种树突状细胞，被鉴定为CD11c(-) CD123（dim+）细胞，被定义为低分化DC，在评估循环DC时必须考虑。此外，过敏儿童，特别是哮喘儿童的DC2计数减少，这可能是靶器官募集增加的结果。
Background Different types of circulating dendritic cells have been described. Dendritic cells influence differentiation of naive T lymphocytes into T helper type 1 (Th1) and Th2 effector cells.Objective The purpose of this study was to evaluate the number of circulating DC subtypes in peripheral blood of allergic and healthy children and in cord blood of neonates from allergic and non-allergic parents.Methods Circulating dendritic cells were flow cytometrically identified in whole blood samples as lineage (CD3, CD14, CD16, CD19, CD20, CD56) negative, CD34 negative and HLA-DR-positive cells. According to the expression of CD123 and CD11c, different DC subtypes were identified.Results Apart from DC1 (CD11c(+) CD123(dim+)) and DC2 (CD11c(-) CD123(high+)), a third DC population was described with less differentiated phenotypic characteristics, namely CD11c(-) CD123(dim+), and therefore defined here as less differentiated DC (ldDC). These ldDC represented the major DC population in cord blood and showed an age-depended decrease. The highest level of ldDC was detected in children with atopic dermatitis, whereas asthmatic children showed the lowest ldDC counts. Furthermore, high-dose inhaled corticosteroid treatment in asthmatic children was related to a decreased ldDC number. The number of circulating DC2 was significantly lower in allergic children, especially in asthmatics, compared to healthy children. In cord blood, no differences in DC subtypes were detectable between neonates at low and high risk for allergic disorders.Conclusion These results indicate that, apart from DC1 and DC2, a third population of dendritic cells, identified as CD11c(-) CD123(dim+) cells and defined as less differentiated DC, must be considered in the evaluation of circulating DC. Furthermore, DC2 counts were decreased in allergic children, especially in asthmatics, which might be the consequence of an increased recruitment to the target organs.