Role of magnesium in the enzyme-linked immunosorbent assay for lipopolysaccharides of rough Escherichia coli strain J5 and Neisseria gonorrhoeae.
Role of magnesium in the enzyme-linked immunosorbent assay for lipopolysaccharides of rough Escherichia coli strain J5 and Neisseria gonorrhoeae.
复制标题
镁在粗大肠杆菌 J5 菌株和淋病奈瑟菌脂多糖酶联免疫吸附测定中的作用。
DOI：
10.1093/infdis/142.4.532
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Braude,AI
中科院分区：
文献类型：
--
作者：
ItoJr,JI;Wunderlich,AC;Lyons,J;Davis,CE;Guiney,DG;Braude,AI
建立了一种检测大肠埃希菌(J5)粗株和淋球菌抗脂多糖(LPS)抗体的酶联免疫吸附试验。将此方法应用于这些LPS，未检测到抗体。用~(51)Cr放射性标记光滑和粗大的内毒素，发现粗大的内毒素不附着在聚苯乙烯管上。然而，当加入镁离子时，所有的内毒素都吸附到固相中。抗核心糖脂抗血清对其光滑亲本株的效价比同源抗血清高，而对淋球菌的抗血清同源效价高，异源效价低。因此，这些数据表明，庞大的O侧链不会阻碍抗体对核心糖脂的渗透，来自淋球菌的LPS是异质的，但确实有交叉反应。
An enzyme-linked immunosorbent assay for antibody to the lipopolysaccharides (LPSs) of a rough strain ofEscherichia coli(J5) andNeisseria gonorrhoeaewas developed. When standard methods of this assay were applied to these LPSs, no antibody was measured. By radiolabeling smooth and rough endotoxins with51Cr, it was discovered that the rough LPS was not adhering to the polystyrene tubes. When Mg++ was added, however, all endotoxins adsorbed to the solid phase. With this assay antiserum to core glycolipid had higher titers against its smooth parent strain than did homologous antiserum, and antisera to gonococci had high homologous but low heterologous titers. Thus, these data demonstrate that bulky O side chains do not hinder the penetration of antibody to core glycolipid and that LPSs from gonococci are heterogeneous but do cross-react.