THE PATH OF CARBON IN PHOTOSYNTHESIS .5. PAPER CHROMATOGRAPHY AND RADIOAUTOGRAPHY OF THE PRODUCTS
THE PATH OF CARBON IN PHOTOSYNTHESIS .5. PAPER CHROMATOGRAPHY AND RADIOAUTOGRAPHY OF THE PRODUCTS
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DOI：
10.1021/ja01160a080
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发表时间：
1950-01-01
影响因子：
15
通讯作者：
STEPKA, W
中科院分区：
文献类型：
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作者：
BENSON, AA;BASSHAM, JA;STEPKA, W
研究植物中二氧化碳的减少需要一种快速和通用的方法来分离和鉴定大量的化合物。纸色谱法就是这样一种方法。本文报道了纸色谱法在植物短时间光合作用中分离化合物实验方法的发展进展。所涉及的化合物是植物和动物代谢的重要中间体。这些方法不仅可以应用于其他生化问题，也可以应用于化学合成的控制。据报道，放射性二氧化碳在短时间的光合作用中形成了许多产物。3-4-6-6-7在这些鉴定中使用的经典生化方法和离子交换技术将需要相当大的努力来分离涉及的大量化合物。纸层析法成功地分离了两组在植物合成中重要的化合物：氨基酸和糖，促使了目前对多羧酸和磷酸酯的分离研究。这些化合物构成了目前已知的合成植物材料的中间体。使用这种方法的首要条件是确定化合物在色谱图中的位置。化学方法和物理方法都用过。前者包括颜色测试，它依赖于使用特定试剂直接在纸上显影颜色：氨基酸用茚三酮，间苯二酚用托伦斯试剂
Investigation of the reduction of carbon dioxide in plants requires a rapid and general method for separating and identifying a large number of com-pounds. Paper chromatography is such a method. This paper constitutes a report of progress in the development of experimental procedures in the application of paper chromatography to the sepa-ration of compounds formed by plants during short periods of photosynthesis. The compounds involved are important intermediates of plant and animal metabolism. These methods should find application not only in other biochemical problems, but in the control of chemical synthesis2 as well. A number of products formed during a short pe-riod of photosynthesis with radioactive carbon di-oxide have been reported. 3-4-6-6-7 The classical biochemical methods and ion exchange techniques which were used in those identifications would re-quire considerable effort to separate the large number of compounds involved. The success of paper chromatography in separating two groups of compounds important inphytosynthesis, amino acids8 and sugars, 9'10 prompted the present work on separation of polycarboxylic acids and phos-phate esters. These compounds constitute the presently known intermediates in synthesis of plant material. 11The primary requisite for the use of this method is some means of determining the position of a compound in the chromatogram. Both chemical and physical methods have been used. The former includes color tests which depend on development of a color directly on the paper by the use of specific reagents: ninhydrin for amino acids, 8naphthoresorcinolor Tollens reagent for sug-