Microtubule-binding protein FOR20 promotes microtubule depolymerization and cell migration.
Microtubule-binding protein FOR20 promotes microtubule depolymerization and cell migration.
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微管结合蛋白 FOR20 促进微管解聚和细胞迁移
DOI：
10.1038/celldisc.2017.32
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发表时间：
2017
期刊：
影响因子：
33.5
通讯作者：
Zhou T
中科院分区：
文献类型：
--
作者：
Feng S;Song Y;Shen M;Xie S;Li W;Lu Y;Yang Y;Ou G;Zhou J;Wang F;Liu W;Yan X;Liang X;Zhou T
微管是由αβ-微管蛋白异源二聚体组装而成的高度动态的细丝，在许多细胞过程中发挥重要作用，包括细胞分裂和迁移。微管动力学受到微管相关蛋白（MAP）的严格调控，MAP通过与微管或游离微管蛋白二聚体结合发挥作用。在这里，我们报告说，FOR 20（FOP相关蛋白的20 kDa），一个保守的蛋白质的纤毛发生和细胞周期的进展至关重要，是一个以前未知的MAP，有利于微管解聚和促进细胞迁移。FOR 20不仅与微管直接结合，而且通过降低微管生长速率、增加解聚速率和突变频率来调节微管动力学。在体外微管动力学分析中，FOR 20似乎优先与微管蛋白二聚体相互作用。在HeLa细胞中，FOR 20的耗尽抑制微管解聚并促进诺考达唑处理后的微管再生长。此外，F0 R20敲低显著抑制哺乳动物细胞的个体和集体迁移。综上所述，这些数据表明，FOR 20作为MAP的功能，以促进微管解聚和细胞迁移。
Microtubules are highly dynamic filaments assembled from αβ-tubulin heterodimers and play important roles in many cellular processes, including cell division and migration. Microtubule dynamics is tightly regulated by microtubule-associated proteins (MAPs) that function by binding to microtubules or free tubulin dimers. Here, we report that FOR20 (FOP-related protein of 20 kDa), a conserved protein critical for ciliogenesis and cell cycle progression, is a previously uncharacterized MAP that facilitates microtubule depolymerization and promotes cell migration. FOR20 not only directly binds to microtubules but also regulates microtubule dynamics in vitro by decreasing the microtubule growth rate and increasing the depolymerization rate and catastrophe frequency. In the in vitro microtubule dynamics assays, FOR20 appears to preferentially interact with free tubulin dimers over microtubules. Depletion of FOR20 inhibits microtubule depolymerization and promotes microtubule regrowth after the nocodazole treatment in HeLa cells. In addition, FOR20 knockdown significantly inhibits both individual and collective migration of mammalian cells. Taken together, these data suggest that FOR20 functions as a MAP to promote microtubule depolymerization and cell migration.
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影响因子：
4
作者：
Bowne-Anderson, Hugo;Zanic, Marija;Kauer, Monika;Howard, Jonathon
通讯作者：
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影响因子：
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DOI：
10.1073/pnas.0703094104
发表时间：
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