Biodegradation of pyrene by Mycobacterium frederiksbergense in a two-phase partitioning bioreactor system.
Biodegradation of pyrene by Mycobacterium frederiksbergense in a two-phase partitioning bioreactor system.
复制标题
DOI：
10.1016/j.biortech.2007.05.042
复制
发表时间：
2008-05
影响因子：
11.4
通讯作者：
B. Mahanty;K. Pakshirajan;V. V. Dasu-V.
中科院分区：
文献类型：
--
作者：
B. Mahanty;K. Pakshirajan;V. V. Dasu-V.
在以硅油为非水相液体(NAPL)的两相分配生物反应器(TPPB)中，研究了弗雷德克斯伯根分枝杆菌对芘的生物降解作用。在活性降解阶段，初始浓度为1000mgl、600mgl、400mgl、200mgl的−1D−1的利用率分别为270mgl、230mgl、139mgl、82mgl−1。在TPPB中使用M.frederksbergense实现的降解率远远高于文献报道的使用单一和纯微生物物种运行的异地多环芳烃生物降解系统的值。降解数据符合简单的Monod、Logistic、对数、三个半级动力学模型。在这些模型中，只有指数增长形式的三个半级动力学模型对整个降解剖面提供了最好的拟合，决定系数(R2)值为>；0.99。从实验结果来看，微生物在TPPB中对Py的吸收是一种基于非界面的机理。
Biodegradation of pyrene by Mycobacterium frederiksbergense was studied in a two-phase partitioning bioreactor (TPPB) using silicone oil as non-aqueous phase liquid (NAPL). The TPPB achieved complete biodegradation of pyrene; and during the active degradation phase, utilization rates of 270, 230, 139, 82mgl−1d−1for initial pyrene loading concentrations (in NAPL) of 1000, 600, 400 and 200mgl−1, respectively, were obtained. The degradation rates achieved using M. frederiksbergense in TPPB were much higher than the literature reported values for an ex situ PAH biodegradation system operated using single and pure microbial species. The degradation data was fitted to simple Monod, logistic, logarithmic, three-half-order kinetic models. Among these models, only exponential growth form of the three-half-order kinetic model provided the best fit to the entire degradation profiles with coefficient of determination (R2) value >0.99. From the experimental findings, uptake of pyrene by the microorganism in TPPB was proposed to be a non-interfacial based mechanism.