Evaluation of dermal thermal damage by multiphoton autofluorescence and second-harmonic-generation microscopy
Evaluation of dermal thermal damage by multiphoton autofluorescence and second-harmonic-generation microscopy
复制标题
DOI：
10.1117/1.2405347
复制
发表时间：
2006-11-01
影响因子：
3.5
通讯作者：
Lin, Sung-Jan
中科院分区：
文献类型：
--
作者：
Lin, Ming-Gu;Yang, Tsung-Lin;Lin, Sung-Jan
我们试图通过使用多光子显微镜来表征皮肤热损伤的程度。结果表明，真皮胶原蛋白和弹性纤维对热损伤的敏感性不同。在形态上，皮肤胶原蛋白在60℃时开始变性，而弹性纤维在65℃时才发生断裂和聚集。随着温度的升高，弹性纤维和胶原纤维的结构都会恶化。二次谐波（SHG）成像有助于识别胶原蛋白的变性温度，而自体荧光（AF）成像可以帮助识别当SHG信号衰减时，组织在更高温度下的结构变化。我们还采用了一种基于真皮af - shg指数（ASID）的比率法来表征皮肤热损伤程度。使用ASID指数可以绕过分析不均匀真皮纤维的困难，并显示真皮胶原蛋白在60摄氏度时开始变性。我们的研究结果表明，随着进一步的发展，多光子显微镜有可能发展成为一种有效的体内成像技术，以监测和表征皮肤热损伤。(c) 2006年光学仪器工程师学会。
We attempt to characterize the degree of skin thermal damage by using multiphoton microscopy to characterize dermal thermal damage. Our results show that dermal collagen and elastic fibers display different susceptibility to thermal injury. Morphologically, dermal collagen starts to denature at 60 degrees C while fracture and aggregation of elastic fibers do not occur until 65 degrees C. With increasing temperatures, the structures of both elastic and collagen fibers deteriorate. While second-harmonic-generation (SHG) imaging is helpful in identifying the denaturation temperature of collagen, autofluorescence (AF) imaging can help to identify the structural alternations of tissue at higher temperatures when SHG signals have decayed. We also employ a ratiometric approach based on the AF-to-SHG index of dermis (ASID) to characterize the degree of dermal thermal damage. Use of the ASID index can bypass the difficulty in analyzing inhomogeneous dermal fibers and show that dermal collagen starts to denature at 60 degrees C. Our results suggest that with additional developments, multiphoton microscopy has potential to be developed into an effective in vivo imaging technique to monitor and characterize dermal thermal damage. (c) 2006 Society of Photo-Optical Instrumentation Engineers.