Triptolide inhibits TGF-β-induced matrix contraction and fibronectin production mediated by human Tenon fibroblasts
Triptolide inhibits TGF-β-induced matrix contraction and fibronectin production mediated by human Tenon fibroblasts
复制标题
雷公藤内酯醇抑制 TGF-β 诱导的人 Tenon 成纤维细胞介导的基质收缩和纤连蛋白产生
DOI：
10.18240/ijo.2018.07.06
复制
发表时间：
2018-07-18
影响因子：
1.4
通讯作者：
Liu, Ye
中科院分区：
文献类型：
--
作者：
Liu, Yang;Liu, Ping-Ping;Liu, Ye
目的：为了确定雷公藤甲素是否影响人Tenon成纤维细胞（HTFs）的收缩性和纤维连接蛋白的产生，方法：将HTFs培养在I型胶原凝胶中，加入或不加入转化生长因子β（TGF-β）和/或雷公藤甲素。胶原凝胶的直径用于测量收缩。免疫印迹分析用于定量肌球蛋白轻链（MLC）磷酸化和整合素表达。激光共聚焦荧光显微镜用于监测肌动蛋白应力纤维的形成。结果：雷公藤内酯醇对TGF-β诱导的胶原凝胶收缩的抑制作用呈剂量依赖性，在3 nmol/L时有统计学意义（P
AIM: To determine if triptolide influences the contractility and fibronectin production in human Tenon fibroblasts (HTFs).METHODS: HTFs were cultured in type I collagen gels with or without transforming growth factor beta (TGF-beta) and/or triptolide. The diameter of the collagen gel was used to measure contraction. Immunoblot analysis was used to quantify myosin light chain (MLC) phosphorylation and integrin expression. Laser confocal fluorescence microscopy was used to monitor the formation of actin stress fibers. Fibronectin production was measured with an enzyme immunoassay.RESULTS: Triptolide inhibition of contraction in TGF-beta-induced collagen gel mediated by HTFs was dose dependent and statistically significant at 3 nmol/L (P