All-Optical Methods to Study Neuronal Function
All-Optical Methods to Study Neuronal Function
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研究神经元功能的全光学方法
DOI：
10.1007/978-1-0716-2764-8_2
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Quicke P
中科院分区：
文献类型：
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作者：
Quicke P
本章的目标是建立一个框架来评估全视神经生理学实验的成像方法。这并不是对荧光指示剂和成像方式的详尽回顾，而是旨在提炼推动两者选择的功能成像原理。科学优先级决定成像策略是基于“最佳荧光指示剂”还是“最佳成像模式”。由于每个因素对荧光预算和随时间变化的荧光变化的信噪比的贡献，第一个因素的选择限制了第二个因素的选择。
The goal of this chapter is to establish a framework to evaluate imaging methodologies for all-optical neurophysiology experiments. This is not an exhaustive review of fluorescent indicators and imaging modalities but rather aims to distill the functional imaging principles driving the choice of both. Scientific priorities determine whether the imaging strategy is based on an “optimal fluorescent indicator” or “optimal imaging modality.” The choice of the first constrains the choice of the second due to each’s contributions to the fluorescence budget and signal-to-noise ratio of time-varying fluorescence changes.