Synergistic effects of interferon γ and tumour necrosis factor α on T84 cell function
Synergistic effects of interferon γ and tumour necrosis factor α on T84 cell function
复制标题
DOI：
10.1136/gut.45.2.191
复制
发表时间：
1999-08-01
期刊：
影响因子：
24.5
通讯作者：
Reenstra, WW
中科院分区：
文献类型：
--
作者：
Fish, SM;Proujansky, R;Reenstra, WW
背景-炎症性肠病（IBD）的特征是慢性肠道炎症和上皮通透性增加。肿瘤坏死因子α（TNF-α）和干扰素γ方法用IFN-γ和TNF-α培养T84细胞，观察跨上皮电阻（TER）、异硫氰酸荧光素（FITC）、葡聚糖通量、短路电流（I-sc）、检测囊性纤维化跨膜传导调节因子（CFTR）蛋白水平、细胞形态、TNF受体基因表达和细胞凋亡。
Background-Inflammatory bowel disease (IBD) is characterised by chronic intestinal inflammation and increased epithelial permeability. Both tumour necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) have been implicated in IBD.Aims-To understand better the effects of these cytokines on epithelial cell function.Methods-T84 cells were cultured with IFN-gamma and TNF-alpha and changes in transepithelial resistance (TER), fluorescein isothiocyanate (FITC) dextran flux, short circuit current (I-sc), cystic fibrosis transmembrane conductance regulator (CFTR) protein levels, cell morphology, TNF receptor gene expression, and apoptosis were assayed.Results-Relative to controls, significant changes (p