Chemical Characterization of Capsular Polysaccharide from Cryptococcus neoformans Serotype A‐D
Chemical Characterization of Capsular Polysaccharide from Cryptococcus neoformans Serotype A‐D
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新型隐球菌血清型 A-D 荚膜多糖的化学特性
DOI：
10.1111/j.1348-0421.1985.tb02962.x
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发表时间：
1985
影响因子：
2.6
通讯作者：
Y. Fukazawa
中科院分区：
文献类型：
--
作者：
R. Ikeda;A. Nishikawa;T. Shinoda;Y. Fukazawa
在对新型隐球菌血清分型的研究中，我们发现新型隐球菌（CBS 132）的典型菌株为 A-D 血清型。在我们的血清分型系统中，该菌株与因子 7 血清（特异于 A 型血清）和因子 8 血清（特异于血清型 D）发生凝集。因此，我们研究了该菌株的抗原荚膜多糖的化学结构。通过乙醇沉淀从培养上清液中获得可溶性荚膜多糖。对 DEAE-纤维素上的多糖进行柱色谱分离得到三个组分（F-1 至 F-3）。在 F-3 级分中发现了主要的抗原活性。 F-3多糖的甲基化分析、受控Smith降解甲基化分析、部分酸水解和其他结构研究表明，该多糖含有比例为7:2:2的甘露糖、木糖和葡萄糖醛酸，并且具有α(1-3)连接的D-吡喃甘露糖苷残基的主链以及β(1-2)-木糖和葡萄糖醛酸的单分支。 F-3多糖中带支链和不带支链的甘露糖残基的比例为4:3，由平均聚合度计算的分子量为46,500道尔顿。这些结果表明，血清型A-D的荚膜多糖的化学结构与血清型A和D的荚膜多糖非常相似，这表明三种血清型多糖分支中单糖的摩尔比和连接模式的微小差异可能是其不同特异性的原因。
During a study of serotyping of Cryptococcus neoformans, we found that the type strain of C. neoformans (CBS 132) was serotype A‐D. This strain agglutinated with both factor 7 serum (specific for serotype A) and factor 8 serum (specific for serotype D) in our serotyping system. Therefore, we investigated the chemical structure of the antigenic capsular polysaccharide of this strain. The soluble capsular polysaccharide was obtained from the culture supernatant fluid by precipitation with ethanol. Column chromatography of the polysaccharide on DEAE‐cellulose yielded three fractions (F‐1 to F‐3). The major antigenic activity was found in the F‐3 fraction. The results obtained by methylation analysis, controlled Smith degradation‐methylation analysis, partial acid hydrolysis, and other structural studies of F‐3 polysaccharide indicated that the polysaccharide contains mannose, xylose, and glucuronic acid at a ratio of 7:2:2, and has a backbone of α(1–3)‐linked D‐mannopyranoside residues with a single branch of β(1–2)‐xylose and glucuronic acid. The ratio of mannose residues with or without a branch in the F‐3 polysaccharide was 4:3 and its molecular weight calculated from the average of the degree of polymerization was 46,500 daltons. These results indicate that the chemical structure of the capsular polysaccharide of serotype A‐D is very similar to those from serotypes A and D, suggesting that small differences in the molar ratio and pattern of linkage of monosaccharides in the branch of the polysaccharides of the three serotypes may be responsible for their different specificities.
通过质子核磁共振波谱法对富含甘露糖的糖蛋白核心寡糖进行连接和序列分析。
影响因子：
2.9
作者：
Cohen,RE;Ballou,CE
通讯作者：
Ballou,CE