An abundant androgen-regulated mRNA in the mouse kidney
An abundant androgen-regulated mRNA in the mouse kidney
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小鼠肾脏中丰富的雄激素调节 mRNA
DOI：
10.1016/0092-8674(79)90170-3
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发表时间：
1979
期刊：
影响因子：
64.5
通讯作者：
W. Held
中科院分区：
文献类型：
--
作者：
J. Toole;N. Hastie;W. Held
我们通过体外翻译雄性小鼠肾脏的mRNA，确定了一个丰富的20,000道尔顿蛋白(KAP)。这种蛋白质是从女性肾脏的mRNA量减少合成的。纯化KAP基因片段，用于核酸杂交研究。雌性和去势雄性的KAP mRNA水平分别比雄性低10倍和200倍。给雌性或被去势的雄性注射睾酮，会导致KAP基因的表达水平恢复到正常的雄性水平。睾丸女性化(TFM)小鼠的KAP mRNA水平比正常雄性小鼠低3倍，并且不受睾酮诱导。除肾脏外，KAP基因在其他组织中未见明显表达，KAP基因重复性的动力学类似于单拷贝DNA。随着对小鼠遗传学知识的迅速扩展，KAP应该被证明在确定调节激素调节基因的诱导性和组织特异性的遗传因素方面是有用的。
We have identified an abundant 20,000 dalton protein (KAP) by in vitro translation of male mouse kidney mRNA. This protein is synthesized in reduced amounts from female kidney mRNA. A KAP cDNA fragment was purified and used for nucleic acid hybridization studies. Females and castrated males have 10 and 200 fold lower levels, respectively, of KAP mRNA relative to males. The administration of testosterone to females or castrated males results in the induction of KAP mRNA to normal male levels. Testicular feminized (Tfm) mice have 3 fold lower levels of KAP mRNA relative to normal males and are not induced by testosterone. KAP mRNA is not found in significant amounts in tissues other than the kidney, and the KAP gene renatures with kinetics similar to single-copy DNA. With the rapidly expanding knowledge of mouse genetics, KAP should prove useful in determining genetic factors which regulate the inducibllity and tissue specificity of a hormonally regulated gene.