A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
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DOI：
10.4067/s0716-97602005000100010
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发表时间：
2005-01-01
影响因子：
6.7
通讯作者：
SILVA, HERMAN
中科院分区：
文献类型：
--
作者：
MEISEL, LEE;FONSECA, BEATRIZ;SILVA, HERMAN
桃已被提出作为落叶树木和蔷薇科植物的基因组模式。RNA分离的优化方案是必要的，以进一步推进在这个模式物种的研究，功能基因组学分析可以进行。在这里，我们提出了一个优化的协议，以快速，有效地纯化高品质的总RNA从桃果实（桃）。从果实组织中分离高质量的RNA通常是困难的，因为大量的多糖和多酚化合物积累在该组织中并与RNA共纯化。在这里，我们证明了一个修改后的版本的方法，用于分离RNA从松树和木本植物细叶肉桂是理想的高品质的RNA从桃的果实分离。该RNA可用于许多基于功能基因组学的实验，如RT-PCR和大插入cDNA文库的构建。
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilion is ideal for isolating high quality RNA from the fruits of Prunus persica. This RNA may be used for many functional-enomic based experiments such as RT-PCR and the construction of large-insert cDNA libraries.