SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex.
SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex.
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SPOP 对于前列腺癌细胞中 DNA-蛋白质交联修复至关重要：SPOP 依赖性地从拓扑异构酶 2A-DNA 裂解复合物中去除拓扑异构酶 2A。
DOI：
10.1091/mbc
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发表时间：
2020
影响因子：
3.3
通讯作者：
et al.
中科院分区：
文献类型：
--
作者：
Watanabe R; Kikugawa T;Saika T;Higashiyama S;et al.
衣原体顺行鞭毛内转运蛋白Kinesin-2是一种由两个运动亚基和一个非运动亚基组成的异三聚体复合体，称为Kinesin相关多肽或KAP。两个马达亚基中的一个由FLA10基因编码。通过质谱分析和测序获得第二个马达亚基的序列。该基因与Fla10马达亚基的同源性为46.9%，基因定位于RPL9附近的XII/XIII连锁群。温度敏感的鞭毛组装突变体FLA1和FLA8与这个Kinesin-2马达亚基相连。在每个菌株中，一个独特的单点突变会导致运动域中唯一的单一氨基酸替代。FLA8菌株命名为FLA8-1，FLA1菌株命名为FLA8-2。FLA8和FLA10等位基因表现为染色体丢失表型。为了分析这种染色体丢失表型，我们产生了LA8-1、FLA8-2和FLA10-14的基因内回复突变体。对突变体和回复突变体的分析表明，这些马达亚基氨基末端的一个口袋对于运动活动和对染色体分离保真度的新的显性效应都很重要。
TheChlamydomonasanterograde intraflagellar transport motor, kinesin-2, is isolated as a heterotrimeric complex containing two motor subunits and a nonmotor subunit known as kinesin-associated polypeptide or KAP. One of the two motor subunits is encoded by theFLA10gene. The sequence of the second motor subunit was obtained by mass spectrometry and sequencing. It shows 46.9% identity with the Fla10 motor subunit and the gene maps to linkage group XII/XIII near RPL9. The temperature-sensitive flagellar assembly mutantsfla1andfla8are linked to this kinesin-2 motor subunit. In each strain, a unique single point mutation gives rise to a unique single amino acid substitution within the motor domain. Thefla8strain is namedfla8-1and thefla1strain is namedfla8-2. Thefla8andfla10alleles show a chromosome loss phenotype. To analyze this chromosome loss phenotype, intragenic revertants offla8-1, fla8-2, andfla10-14were generated. The analysis of the mutants and the revertants demonstrates the importance of a pocket in the amino terminus of these motor subunits for both motor activity and for a novel, dominant effect on the fidelity of chromosome segregation.