Responses of N2O production pathways and related functional microbes to temperature across greenhouse vegetable field soils
Responses of N2O production pathways and related functional microbes to temperature across greenhouse vegetable field soils
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DOI：
10.1016/j.geoderma.2019.113904
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发表时间：
2019-12-01
期刊：
影响因子：
6.1
通讯作者：
Xiong, Zhengqin
中科院分区：
文献类型：
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作者：
Duan, Pengpeng;Song, Yanfeng;Xiong, Zhengqin
温室菜地是农业一氧化二氮（N2O）排放的主要来源之一，通过不同微生物调节的多种途径。然而，不同温室蔬菜土壤中硝化菌硝化作用、硝化菌反硝化作用、硝化耦合反硝化作用和异养反硝化作用对N2O产生随温度变化的相对贡献却知之甚少。本研究采用N-15-O-18标记技术和转录组分析相结合的方法，研究了中国大陆6个温室菜田土壤在15、25和35℃下N2O产生途径（硝化菌硝化、硝化菌反硝化、硝化耦合反硝化和异养反硝化）。结果表明，异养反硝化作用是酸性温室蔬菜土壤中N2O的重要来源，而硝化菌硝化作用是碱性温室蔬菜土壤中N2O的主要来源。此外，碱性温室蔬菜土壤中硝化菌反硝化和硝化耦合反硝化对N2O产生的贡献高于酸性温室蔬菜土壤。在15-35℃温度范围内，硝化耦合反硝化作用的贡献随着温度的增加而增加，而硝化细菌反硝化作用随着温度在15-25℃范围内的增加而增加。总体而言，高温降低了硝化细菌硝化作用对N2O产生的贡献。这些结果对于了解温度依赖性 N2O 生产途径以及减少不同土壤和温度条件下农业 N2O 排放具有重要意义。
Greenhouse vegetable field is one of the main sources of agricultural nitrous oxide (N2O) emissions via multiple pathways regulated by different microbes. However, the relative contributions of nitrifier nitrification, nitrifier denitrification, nitrification-coupled denitrification and heterotrophic denitrification to N2O production with temperature change in different greenhouse vegetable soils are poorly understood. In this study, combined approaches of the N-15-O-18 labeling technique and transcriptome analyses were applied to investigate the pathways of N2O production (nitrifier nitrification, nitrifier denitrification, nitrification-coupled denitrification and heterotrophic denitrification) under 15, 25 and 35 degrees C in six greenhouse vegetable field soils in mainland China. The results show that heterotrophic denitrification was an important source of N2O in acidic greenhouse vegetable soils, whereas nitrifier nitrification was the dominant source of N2O in alkaline greenhouse vegetable soils. In addition, the contribution of nitrifier denitrification and nitrification-coupled denitrification to N2O production in alkaline greenhouse vegetable soils were higher than in acidic greenhouse vegetable soils. The contribution of nitrification-coupled denitrification increased with temperature at 15-35 degrees C, whereas nitrifier denitrification increased with temperature at 15-25 degrees C. Overall, high temperature decreased the contribution of nitrifier nitrification to N2O production. These results have important implications for understanding the temperature-dependent N2O production pathways and for mitigating agricultural N2O emissions under different soil and temperature conditions.