Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities
Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities
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DOI：
10.1016/j.biortech.2007.09.064
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发表时间：
2008-07-01
影响因子：
11.4
通讯作者：
Decker, Stephen R.
中科院分区：
文献类型：
--
作者：
Selig, Michael J.;Knoshaug, Eric P.;Decker, Stephen R.
使用少量的非纤维素分解细胞壁降解酶，观察到纤维二糖水解酶I（Cel 7A）从里氏木霉玉米秸秆纤维素的解聚显着增加。纯化的内切木聚糖酶（XynA）、阿魏酸酯酶（FaeA）和乙酰木聚糖酯酶（阿克塞尔）都增强了Cel 7A对经受热水预处理的玉米秸秆的性能。在所有情况下，这些活性的添加提高了酶水解的有效性，以每毫克总蛋白转化的纤维素的量计。通过添加非纤维素分解酶对纤维二糖释放的改善范围为比单独的Cel 7A增加13-84%。最有效的组合包括添加XynA和阿克塞尔两者，其协同增强木聚糖转化，导致葡聚糖转化的额外协同改进。此外，我们注意到在XynA的存在下酶促木聚糖去除和通过Cel 7A增强纤维素水解之间的直接关系。(C)2007爱思唯尔有限公司保留所有权利。
Significant increases in the depolymerization of corn stover cellulose by cellobiohydrolase I (Cel7A) from Trichoderma reesei were observed using small quantities of non-cellulolytic cell wall-degrading enzymes. Purified endoxylanase (XynA), ferulic acid esterase (FaeA), and acetyl xylan esterase (Axel) all enhanced Cel7A performance on corn stover subjected to hot water pretreatment. In all cases, the addition of these activities improved the effectiveness of the enzymatic hydrolysis in terms of the quantity of cellulose converted per milligram of total protein. Improvement in cellobiose release by the addition of the non-cellulolytic enzymes ranged from a 13-84% increase over Cel7A alone. The most effective combinations included the addition of both XynA and Axel, which synergistically enhance xylan conversions resulting in additional synergistic improvements in glucan conversion. Additionally, we note a direct relationship between enzymatic xylan removal in the presence of XynA and the enhancement of cellulose hydrolysis by Cel7A. (C) 2007 Elsevier Ltd. All rights reserved.