Patterns of intracellular calcium fluctuation in precursor cells of the neocortical ventricular zone
Patterns of intracellular calcium fluctuation in precursor cells of the neocortical ventricular zone
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DOI：
10.1523/jneurosci.18-14-05374.1998
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发表时间：
1998-07-15
影响因子：
5.3
通讯作者：
Kriegstein, AR
中科院分区：
文献类型：
--
作者：
Owens, DF;Kriegstein, AR
细胞内游离钙浓度([Ca~(2+)](I))的变化被认为会影响发育中的神经元的各种事件。虽然在未成熟的皮质神经元中已经检测到[Ca~(2+)](I)的自发变化，但皮质前体细胞的钙动力学还没有得到足够的重视。利用完整的皮层外套膜和激光共聚焦显微镜，我们在原位观察了新皮质脑室带(VZ)细胞自发性[Ca~(2+)](I)波动的时空模式。大多数活性由单个细胞组成，表现出独立的[Ca~(2+)](I)波动。这些事件发生在整个VZ深度的细胞中。免疫组织化学染色证实，这些事件主要发生在前体细胞，而不是有丝分裂后神经元。当成像靠近脑室表面时，常可观察到相邻细胞对的自发性[Ca~(2+)](I)同步升高，细胞形态、时间推移成像和核染色显示这种活动发生在有丝分裂活跃的细胞中。第三种罕见的活动模式包括相邻VZ细胞组中[Ca~(2+)](I)的协同自发增加。这些细胞的形态特征和免疫组织化学染色表明，这些协调事件发生在缝隙连接偶联的前体细胞中。这三种活性模式都依赖于细胞内钙离子的释放。这些结果显示了皮质前体细胞自发的[Ca~(2+)](I)变化的不同模式，并提出了这些动力学可能有助于神经发生的调节。
Changes in intracellular free calcium concentration ([Ca2+](i)) are known to influence a variety of events in developing neurons. Although spontaneous changes of [Ca2+](i) have been examined in immature cortical neurons, the calcium dynamics of cortical precursor cells have received less attention. Using an intact cortical mantle and confocal laser microscopy, we examined the spatiotemporal patterns of spontaneous [Ca2+](i) fluctuations in neocortical ventricular zone (VZ) cells in situ. The majority of activity consisted of single cells that displayed independent [Ca2+](i) fluctuations. These events occurred in cells throughout the depth of the VZ. Immunohistochemical staining confirmed that these events occurred primarily in precursor cells rather than in postmitotic neurons. When imaging near the ventricular surface, synchronous spontaneous [Ca2+](i) increases were frequently observed in pairs of adjacent cells, Cellular morphology, time-lapse imaging, and nuclear staining demonstrated that this activity occurred in mitotically active cells. A third and infrequently encountered pattern of activity consisted of coordinated spontaneous increases in [Ca2+](i) in groups of neighboring VZ cells. The morphological characteristics of these cells and immunohistochemical staining suggested that the coordinated events occurred in gap junction-coupled precursor cells. All three patterns of activity were dependent on the release of Ca2+ from intracellular stores. These results demonstrate distinct patterns of spontaneous [Ca2+](i) change in cortical precursor cells and raise the possibility that these dynamics may contribute to the regulation of neurogenesis.