The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine
The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine
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DOI：
10.1007/7651_2016_15
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Grabacka, Maja
中科院分区：
文献类型：
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作者：
Pierzchalska, Malgorzata;Panek, Malgorzata;Grabacka, Maja
The intestinal epithelium isolated from chicken embryos in last 3 days of development can be used to establish the 3D culture of intestinal organoids. When fragments of epithelial tissue released by incubation with EGTA (2.5 mM, 2 h) are embedded in Matrigel matrix on cell culture inserts the formation of empty spheres covered by epithelial cells is observed in first 24 h of culture. The growth and survival of organoids are supported by the addition of R-spondin 1, Noggin, and prostaglandin E2 to the culture medium. The organoids are accompanied by myofibroblasts which become visible in the next 2 days of culture. The intestinal enteroids (free of myofibroblasts) can be obtained from adult chicken intestine.
