Effects of bisphenol A and nanoscale and microscale polystyrene plastic exposure on particle uptake and toxicity in human Caco-2 cells
Effects of bisphenol A and nanoscale and microscale polystyrene plastic exposure on particle uptake and toxicity in human Caco-2 cells
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双酚 A 以及纳米级和微米级聚苯乙烯塑料暴露对人类 Caco-2 细胞颗粒摄取和毒性的影响
DOI：
10.1016/j.chemosphere.2020.126788
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发表时间：
2020-09-01
期刊：
影响因子：
8.8
通讯作者：
Gao, Zhixian
中科院分区：
文献类型：
--
作者：
Wang, Qiangqiang;Bai, Jialei;Gao, Zhixian
微塑料大量存在于海洋、湖泊、土壤甚至空气中，可通过食物或呼吸道摄入对人类健康构成潜在威胁。而且，微塑料在吸收有机污染物后，对人体具有协同毒性。本研究采用激光扫描共聚焦显微镜和流式细胞术观察结肠癌Caco-2细胞对五种不同粒径（300 nm、500 nm、1 μ m、3 μ m、6 μ m）的聚苯乙烯塑料的摄入情况。不同粒径的微塑料的吸收率分别为73%、71%、49%、43%和30%。采用高效液相色谱法分析了不同粒径聚苯乙烯塑料对双酚A的吸附差异。最后比较了不同粒径的聚苯乙烯微塑料吸附BPA前后对Caco-2细胞的增殖毒性。MTT实验证实，微塑料引起细胞毒性增加。这一结果可能与细胞氧化应激和线粒体去极化增加有关。这一假设已在活性氧（ROS）和线粒体膜电位（MMP）测定中得到证实，因为纳米级微塑料在微塑料暴露后会导致Caco-2细胞上产生大量ROS，而微米级微塑料会导致MMP显著降低。同时，纳米级微塑料由于其对BPA的大比表面积吸附，可引起线粒体进一步去极化，导致微塑料吸附BPA后细胞毒性增强。该研究结果对评价微塑料在人体内的安全性具有重要意义。(C)2020爱思唯尔有限公司保留所有权利。
Microplastics are abundant in oceans, lakes, soils and even air, and can pose potential threats to human health through food or respiratory intake. Moreover, microplastics have synergistic toxicity to the body after absorbing organic pollutants. In this study, laser scanning confocal microscope and flow cytometry were used to observe the intake of colonic cancer Caco-2 cells to polystyrene plastic with five different particlesizes (300 nm, 500 nm, 1 mu m, 3 mu m, 6 mu m). The uptake rates of microplastics with different particle sizes were 73%, 71%, 49%, 43%, and 30%, respectively. Then, High Performance Liquid Chromatography (HPLC) was used to analyze the adsorption differences of polystyrene plastic with different particle sizes to bisphenol A (BPA). Finally, the proliferation toxicity of polystyrene microplastics with different particle sizeson Caco-2 cells before and after adsorption of BPA was compared. MTT experiments confirmed that microplastics caused an increase in cytotoxicity. This result may be related to increased cellular oxidative stress and mitochondrial depolarization. This hypothesis has been confirmed in reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) assays because nanoscale microplastics cause a large amount of ROS on Caco-2 cells after microplastic exposure, and micron-scale microplastics cause a significant decrease in MMP. At the same time, nanoscale micro-plastics can cause further depolarization of mitochondria due to their large specific surface area adsorption of BPA, which leads to enhanced cytotoxicity of microplastics after BPA adsorption. The results of this study are of great significance in the evaluation of the safety of microplastics in the human body. (C) 2020 Elsevier Ltd. All rights reserved.