Blastema induction in aneurogenic state and Prrx-1 regulation by MMPs and FGFs in Ambystoma mexicanum limb regeneration
Blastema induction in aneurogenic state and Prrx-1 regulation by MMPs and FGFs in Ambystoma mexicanum limb regeneration
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DOI：
10.1016/j.ydbio.2011.04.017
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发表时间：
2011-07-15
影响因子：
2.7
通讯作者：
Satou, Yutaka
中科院分区：
文献类型：
--
作者：
Satoh, Akira;Makanae, Aki;Satou, Yutaka
有尾两栖动物可以再生截肢。人们认为，分化的真皮组织在肢体再生过程中产生多能和未分化的细胞，称为胚基细胞。在肢体再生的早期阶段，芽基细胞由神经和顶端上皮帽（AEC）诱导。我们以前调查的作用，神经营养因子在芽或芽样形成组成的Pre-1阳性细胞。一个新的系统，适用于调查早期阶段的肢体再生，称为副肢体模型（ALM），最近开发的。在这项研究中，我们进行了比较转录组分析之间的芽基和伤口使用ALM。基质金属蛋白酶（MMP）和成纤维细胞生长因子（FGF）的信号传导成分被观察到主要在ALM芽基细胞中表达。此外，我们发现，MMP活性诱导芽基标记基因，Pramphin-1，在体外，和FGF信号通路的协调工作，以维持Pramphin-1的表达和ALM芽基的形成。此外，我们证明了这两种活性足以在体内神经不存在的情况下诱导ALM芽基。(C)2011 Elsevier Inc. All rights reserved.
Urodele amphibians can regenerate amputated limbs. It has been considered that differentiated dermal tissues generate multipotent and undifferentiated cells called blastema cells during limb regeneration. In early phases of limb regeneration, blastema cells are induced by nerves and the apical epithelial cap (AEC). We had previously investigated the role of neurotrophic factors in blastema or blastema-like formation consisting of Prrx-1 positive cells. A new system suitable for investigating early phases of limb regeneration, called the accessory limb model (ALM), was recently developed. In this study, we performed a comparative transcriptome analysis between a blastema and wound using ALM. Matrix metalloproteinase (MMP) and fibroblast growth factor (FGF) signaling components were observed to be predominantly expressed in ALM blastema cells. Furthermore, we found that MMP activity induced a blastema marker gene, Prrx-1, in vitro, and FGF signaling pathways worked in coordination to maintain Prrx-1 expression and ALM blastema formation. Furthermore, we demonstrated that these two activities were sufficient to induce an ALM blastema in the absence of a nerve in vivo. (C) 2011 Elsevier Inc. All rights reserved.