Reversible blockade of rodent whisking: Botulinum toxin as a tool for developmental studies.
Reversible blockade of rodent whisking: Botulinum toxin as a tool for developmental studies.
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可逆阻断啮齿动物搅拌：肉毒杆菌毒素作为发育研究的工具。
DOI：
10.1080/0899022021000037818
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Zeigler,HPhilip
中科院分区：
文献类型：
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作者：
Landers,Margo;Pytte,Carolyn;Zeigler,HPhilip
对啮齿动物的扭动系统等感觉运动系统的研究表明，在发育过程中身体运动及其感觉结果之间的联系可能对该系统的功能组织做出重要贡献。在本研究中，我们探索了肉毒杆菌毒素在搅拌的发育研究中的可能用途。肉毒杆菌选择性地阻断由搅拌产生的传入，而不影响其他胡须传入来源。我们描述了适当的注射方式，定义了剂量水平，并评估了发育过程中长期的扭动麻痹对成年大鼠基本运动能力的影响。我们的发现表明：(A)肉毒杆菌毒素可用于阻止成年和发育中的大鼠的扭动行为，(B)肉毒杆菌毒素阻断在发育中和成年动物中产生的扭动麻痹的持续时间与剂量有关，(C)这种阻断在功能上是可逆的，(D)发育期间的肉毒杆菌治疗不会损害成年扭动行为的运动学或节律性模式。肉毒杆菌毒素可能是一种有用的工具，可以用来研究经验因素在啮齿动物“主动触摸”发展过程中的作用。
Studies of sensorimotor systems such as the whisking system of rodents have suggested that associations between body movements and their sensory consequences during development may make an important contribution to the functional organization of the system. In the present study we have explored the possible utility of Botulinum toxin for developmental studies of whisking. Botox selectively blocks whisking-generated afference leaving other sources of whisker afference intact. We describe appropriate modes of injection, define dosage levels, and assess the effects of prolonged whisking paralysis during development upon the basic motor competency of the adult rat. Our findings indicate that: (a) Botulinum toxin may be used to block whisking behavior in adult and developing rats, (b) that the duration of the whisking paralysis produced by Botox treatment blockade is dose dependent in both developing and adult animals, (c) that the blockade is functionally reversible and (d) that Botox treatment during development does not impair either the kinematics or the rhythmic patterning of adult whisking behavior. Botox may be a useful tool for studying the role of experiential factors in the development of "active touch" in rodents.