A putative molybdate transporter LjMOT1 is required for molybdenum transport in Lotus japonicus
A putative molybdate transporter LjMOT1 is required for molybdenum transport in Lotus japonicus
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DOI：
10.1111/ppl.12489
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发表时间：
2016-11-01
影响因子：
6.4
通讯作者：
Lin, Yi
中科院分区：
文献类型：
--
作者：
Gao, Jun-Shan;Wu, Fei-Fei;Lin, Yi
钼（Mo）是植物生长发育所必需的微量元素，影响豆科植物根瘤的形成和固氮作用。在这项研究中，百脉根生长在MS固体培养基上含有0 nmol l（-1）（-Mo），103 nmol l（-1）（+Mo）和1030 nmol l（-1）（10 x Mo）的Mo。在3种不同培养基上生长15 d后，植株的表型没有明显差异，但在-Mo上生长45 d后，植株出现典型的缺钼症状，如主根短、侧根少和叶片变黄。从L. - 是的该基因编码468个氨基酸，包括两个保守的基序，并被预测定位于L.染色体组。同源性比较表明，LjMOT 1与其他MOT 1蛋白具有较高的相似性，与GmMOT 1有较近的亲缘关系。亚细胞定位表明LjMOT 1定位于质膜。qRT-PCR分析表明，钼浓度的增加调节了LjMOT 1的相对表达水平。此外，钼浓度在地上部与LjMOT 1的表达量呈正相关，而在根中相关性不明显。此外，硝酸还原酶活性的变化与钼浓度的变化一致。这些结果表明，LjMOT 1可能参与了Mo的运输，为进一步了解Mo在高等植物中的运输机制提供了理论依据。
Molybdenum (Mo) is an essential micronutrient that is required for plant growth and development, and it affects the formation of root nodules and nitrogen fixation in legumes. In this study, Lotus japonicus was grown on MS solid media containing 0 nmol l(-1) (-Mo), 103 nmol l(-1) (+Mo) and 1030 nmol l(-1) (10 x Mo) of Mo. The phenotypes of plants growing on the three different media showed no obvious differences after 15 days, but the plants growing on -Mo for 45 days presented typical symptoms of Mo depletion, such as a short taproot, few lateral roots and yellowing leaves. A Mo transporter gene, LjMOT1, was isolated from L. japonicus. It encoded 468 amino acids, including two conserved motifs, and was predicted to locate to chromosome 3 of the L. japonicus genome. A homology comparison indicated that LjMOT1 had high similarities to other MOT1 proteins and was closely related to GmMOT1. Subcellular localization indicated that LjMOT1 is localized to the plasma membrane. qRT-PCR analyses showed that increasing Mo concentrations regulated the relative expression level of LjMOT1. Moreover, the Mo concentration in shoots was positively correlated to the expression of LjMOT1, but there was no such evident correlation in the roots. In addition, changes in the nitrate reductase activity were coincident with changes in the Mo concentration. These results suggest that LjMOT1 may be involved in the transport of Mo and provide a theoretical basis for further understanding of the mechanism of Mo transport in higher plants.