TRANSFER-RNA PUNCTUATION MODEL OF RNA PROCESSING IN HUMAN MITOCHONDRIA
TRANSFER-RNA PUNCTUATION MODEL OF RNA PROCESSING IN HUMAN MITOCHONDRIA
复制标题
DOI：
10.1038/290470a0
复制
发表时间：
1981-01-01
期刊：
影响因子：
64.8
通讯作者：
ATTARDI, G
中科院分区：
文献类型：
--
作者：
OJALA, D;MONTOYA, J;ATTARDI, G
对HeLa细胞线粒体DNA重链中编码的大多数推定mRNA的3“-末端近端片段进行部分测序，并与DNA序列进行比对。在所有情况下，单个mRNA编码序列的3“-末端核苷酸紧邻tRNA基因或另一种mRNA编码序列。编码rRNA、含poly（A）的RNA和tRNA物质的重（H）链序列显然形成了在该链的几乎整个长度上延伸的连续体。H链显然被转录成单个多顺反子RNA分子，随后通过精确的核酸内切裂解将其加工成成熟的种类，在大多数情况下，核酸内切裂解发生在紧接tRNA序列之前和之后。
A 3''-end proximal segment of most of the putative mRNA encoded in the heavy strand of HeLa cell mt[mitochondrial]DNA was partially sequenced and aligned with the DNA sequence. In all cases, the 3''-end nucleotide of the individual mRNA coding sequences was immediately contiguous to a tRNA gene or another mRNA coding sequence. The heavy (H) strand sequences coding for the rRNA, poly(A)-containing RNA and tRNA species apparently form a continuum extending over almost the entire length of this strand. The H strand apparently is transcribed into a single polycistronic RNA molecule, which is processed later into mature species by precise endonucleolytic cleavages which occur, in most cases, immediately before and after a tRNA sequence.