Maintenance of high extracellular pH does not influence cell pH or metabolism in submerged anoxic bullfrogs.
Maintenance of high extracellular pH does not influence cell pH or metabolism in submerged anoxic bullfrogs.
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维持高细胞外 pH 值不会影响水下缺氧牛蛙的细胞 pH 值或新陈代谢。
DOI：
10.1002/jez.1402650603
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Warburton,SJ
中科院分区：
文献类型：
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作者：
Wasser,JS;Jackson,DC;Chang,SY;Warburton,SJ
我们比较了瘫痪牛蛙在15°C下缺氧浸没4小时后细胞外和细胞内的酸碱状态，通过碳酸氢盐输注将细胞外pH值维持在缺氧前水平。我们还通过测量肝脏、心脏和骨骼肌中的组织乳酸盐和糖原浓度来评估这些条件下的无氧代谢。虽然碳酸氢盐输注导致动脉血pH（pHe）显著高于盐水输注，但心肌和骨骼肌细胞内pH（pHi）在缺氧4 h后无显著差异。我们也无法证明无氧代谢率的任何差异，因为在所研究的组织中，碳酸氢盐和盐水输注青蛙的组织乳酸盐蓄积和糖原消耗相同。我们得出结论：（1）碳酸氢盐输注引起的细胞外酸碱状态的改变不一定反映在细胞内室中，这可能是由于强大的细胞内缓冲过程，以及（2）牛蛙缺氧期间维持碱性细胞外pH不会影响无氧代谢率。然而，我们不能排除细胞内pH值在调节青蛙缺氧过程中的无氧代谢中可能发挥的作用。© 1993 Wiley利斯公司
We compared extracellular and intracellular acid‐base states in paralyzed bullfrogs subjected to 4 h of anoxic submergence at 15°C with or without maintenance of extracellular pH at preanoxic levels by bicarbonate infusion. We also assessed anaerobic metabolism under these conditions by measuring tissue lactate and glycogen concentrations in liver, heart, and skeletal muscle. Although bicarbonate infusion resulted in a significantly higher arterial blood pH (pHe) than saline infusion, intracellular pH (pHi) of heart and skeletal muscle, as determined by the DMO equilibration technqiue, were not significantly different after 4 h of anoxia. We were also unable to demonstrate any differences in anaerobic metabolic rate, since both tissue lactate accumulation and glycogen depletion were identical in bicarbonate‐ and saline‐infused frogs in the tissues studied. We conclude that (1) alterations in the extracellular acid‐base state by bicarbonate infusion are not necessarily reflected in the intracellular compartment, perhaps due to powerful intracellular buffering processes, and (2) maintenance of an alkaline extracellular pH during anoxia in bullfrogs does not influence the anaerobic metabolic rate. We could not, however, rule out a possible role for intracellular pH in regulating anaerobic metabolism during anoxia in frogs. © 1993 Wiley‐Liss, Inc.