Laminin-5 expression is independent of the injury and the microenvironment during reepithelialization of wounds
Laminin-5 expression is independent of the injury and the microenvironment during reepithelialization of wounds
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DOI：
10.1177/002215549804600309
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发表时间：
1998-03-01
影响因子：
3.2
通讯作者：
Oikarinen, A
中科院分区：
文献类型：
--
作者：
Kainulainen, T;Häkkinen, L;Oikarinen, A
我们研究了层粘连蛋白-5及其整合素受体在人类伤口再上皮化过程中的表达。我们使用皮肤的抽吸水泡作为角质形成细胞在基底膜基质上迁移的模型，并使用粘膜全层伤口作为角质形成细胞在临时基质中迁移的模型。将人表皮角质形成细胞注射到无胸腺小鼠背部的动物模型用于跟踪基底膜组分的沉积。在4日龄水疱中，迁移舌前缘约20-50个细胞显示细胞质层粘连蛋白-5免疫染色。层粘连蛋白-5mRNA在迁移表皮前缘的15-30个细胞中检测到。α（3）β（1）和α（6）β（4）整联蛋白在迁移的基底细胞的膜突起中以及在基底上细胞层中被发现，表明它们在结合层粘连蛋白-5中的组合作用。在粘膜伤口中，层粘连蛋白-5是沉积在凝块和迁移的角质形成细胞之间的唯一基底膜区成分。在动物模型中，层粘连蛋白-5和alpha（6）beta（4）整联蛋白的线性沉积在第2天就已观察到，而其他基底膜区成分尚未形成。结果表明，无论损伤和微环境如何，层粘连蛋白-5在伤口角质形成细胞和周围基质之间的相互作用中起着至关重要的作用。
We examined the expression of laminin-5 and its integrin receptors during reepithelialization of human wounds. We used suction blisters of skin as a model of keratinocyte migration on a basement membrane matrix and mucosal full-thickness wounds as a model in which keratinocytes migrate in a provisional matrix. An animal model, in which human epidermal keratinocytes were injected into the back of athymic mice, was used to follow the deposition of the basement membrane components. In 4-day-old blisters, about 20-50 cells at the leading edge of the migrating tongue showed cytoplasmic laminin-5 immunostaining. Laminin-5 mRNA was detected in 15-30 cells at the leading edge of the migrating epidermis. alpha(3) beta(1) and alpha(6) beta(4) integrins were found in membrane projections of the migrating basal cells and also in suprabasal cell layers, suggesting their combined role in binding laminin-5. In mucosal wounds, laminin-5 was the only basement membrane zone component that was deposited between the clot and the migrating keratinocytes. In the animal model, linear deposition of laminin-5 and alpha(6) beta(4) integrin was already seen on Day 2, whereas the other basement membrane zone components were not yet organized. The results suggest that, regardless of the injury and the microenvironment, laminin-5 plays an essential role in the interaction between wound keratinocytes and the surrounding matrix.