Effect of electrical stimulation combined with craphene-oxide-based membranes on neural stem cell proliferation and differentiation
Effect of electrical stimulation combined with craphene-oxide-based membranes on neural stem cell proliferation and differentiation
复制标题
电刺激联合氧化紫薇膜对神经干细胞增殖和分化的影响
DOI：
10.1080/21691401.2019.1613422
复制
发表时间：
2019-01-01
影响因子：
5.8
通讯作者：
Yang, Xiaoyu
中科院分区：
文献类型：
--
作者：
Fu, Chuan;Pan, Su;Yang, Xiaoyu
利用可降解高分子材料与生物或物理因子复合制备的复合神经材料作为治疗神经损伤的手段受到广泛关注。本研究旨在探讨氧化石墨烯（GO）复合导电材料结合电刺激（ES）在神经修复中的潜在应用。制备了聚乳酸-羟基乙酸共聚物（PLGA）IGO导电复合膜，并利用扫描电镜、接触角仪和力学性能测试仪对其性能进行了测试。接着，将神经干细胞（NSC）种植在PLGA/GO导电复合膜上并施加ES。分别用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物（KIT）法、免疫荧光法和PCR法观察神经干细胞增殖、分化和轴突伸长。结果表明，PLGA/GO膜具有良好的亲水性、机械强度和蛋白质吸附性能。ES与PLGA/GO膜结合显著促进材料表面的NSC增殖和神经元分化，并促进显著的神经突起伸长。我们的研究结果表明，ES结合GO相关的导电复合材料可以作为一种新的治疗组合来治疗神经损伤。
The combination of composite nerve materials prepared using degradable polymer materials with biological or physical factors has received extensive attention as a means to treat nerve injuries. This study focused on the potential application of graphene oxide (GO) composite conductive materials combined with electrical stimulation (ES) in nerve repair. A conductive poly(L-lactic-co-glycolic acid) (PLGA)IGO composite membrane was prepared, and its properties were tested using a scanning electron microscope (SEM), a contact angle meter, and a mechanical tester. Next, neural stem cells (NSCs) were planted on the PLGA/GO conductive composite membrane and ES was applied. NSC proliferation and differentiation and neurite elongation were observed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (KIT) assay, immunofluorescence, and PCR, respectively. The results showed that the PLGA/GO membrane had good hydrophilicity, mechanical strength, and protein adsorption. ES combined with the PLGA/GO membrane significantly promoted NSC proliferation and neuronal differentiation on the material surface and promoted significant neurite elongation. Our results suggest that ES combined with GO-related conductive composite materials can be used as a new therapeutic combination to treat nerve injuries.