Dynamic interactions between microtubules and artificial membranes.
Dynamic interactions between microtubules and artificial membranes.
复制标题
微管和人造膜之间的动态相互作用。
DOI：
10.1021/bi00386a063
复制
发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Berlin,RD
中科院分区：
文献类型：
--
作者：
Caron,JM;Berlin,RD
Materials and Methods Protein Isolation and Labeling Procedures. Microtubule protein was prepared from bovine, brain by three cycles of assembly-disassembly according to the procedure of Margolis and Wilson (1978) with slight modifications (Regula et al., 1981). Microtubule-associated proteins (MAPs) and tubulin represented approximately 20% and 80% of the protein, re-spectively, as determined by SDS-polyacrylamide gelelectrophoresis and scanning densitometry. Protein concentrations were determined by the method of Lowry (1951). The critical concentration (Cc) for microtubule assembly was determined as previously described (Regula et al., 1981). Microtubule protein was labeled with the fluorescent dye (dichlorotriazino) aminofluorescein (DTAF)(Molecular fThis work was supported by Grant CA15544 to RDB from the National Institutes of Health.
