THE INTERACTION OF TRYPANOSOMA-CONGOLENSE WITH ENDOTHELIAL-CELLS
THE INTERACTION OF TRYPANOSOMA-CONGOLENSE WITH ENDOTHELIAL-CELLS
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DOI：
10.1017/s0031182000076514
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发表时间：
1994-12-01
期刊：
影响因子：
2.4
通讯作者：
ROSS, CA
中科院分区：
文献类型：
--
作者：
HEMPHILL, A;FRAME, I;ROSS, CA
研究了影响培养的刚果锥虫血流型与哺乳动物饲养细胞粘附的因素。使用体外结合试验，锥虫与牛主动脉内皮（BAE）细胞相互作用后的初始事件进行了监测，通过光学和电子显微镜代谢抑制剂和其他生化物质与细胞或寄生虫孵育，以测试是否有任何抑制的过程。我们的研究结果表明，寄生虫的粘附是一个积极的过程，需要代谢能量的锥虫，但不是从内皮细胞。我们还提供了数据表明，T。刚果人的血流形式具有定位于其鞭毛表面上不同位点的凝集素样结构域，其与内皮细胞质膜上的特异性碳水化合物受体（最可能是唾液酸残基）相互作用。我们还建议，细胞骨架蛋白肌动蛋白可能参与这种相互作用。
Factors which affect adhesion of cultured Trypanosoma congolense bloodstream forms to mammalian feeder cells have been examined. Using an in vitro binding assay, the initial events following interaction of trypanosomes with bovine aorta endothelial (BAE) cells were monitored by both light- and electron microscopy metabolic inhibitors and other biochemicals were incubated with either cells or parasites, to test whether any inhibited the process. Our findings suggest that adhesion of the parasites is an active process requiring metabolic energy from the trypanosomes, but not from endothelial cells. We also provide data suggesting that T. congolense bloodstream forms possess a lectin-like domain, localized at distinct sites on their flagellar surface, which interacts with specific carbohydrate receptors, most likely sialic acid residues, on the endothelial cell plasma membrane. We also suggest that the cytoskeletal protein actin is probably involved in this interaction.