Recombinant Pfs25 protein of Plasmodium falciparum elicits malaria transmission-blocking immunity in experimental animals.
Recombinant Pfs25 protein of Plasmodium falciparum elicits malaria transmission-blocking immunity in experimental animals.
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DOI：
10.1084/jem.174.5.1203
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发表时间：
1991-11-01
期刊：
影响因子：
--
通讯作者：
Kaslow DC
中科院分区：
文献类型：
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作者：
Barr PJ;Green KM;Gibson HL;Bathurst IC;Quakyi IA;Kaslow DC
Pfs 25是恶性疟原虫的有性阶段抗原，其表达于寄生虫的合子和动合子形式的表面上。针对天然Pfs 25的单克隆抗体可以完全阻断恶性疟原虫卵囊在蚊子载体中肠中的发育。因此，这种25 kD的蛋白质是一个潜在的疫苗候选人引发疟疾流行地区的居民的传播阻断免疫。我们已经通过从酵母分泌合成了Pfs 25的多肽类似物，当与胞壁酰三肽佐剂一起用于免疫小鼠和猴时，其与构象依赖性单克隆抗体和elastinase传递阻断抗体反应。我们的研究结果表明，进一步评估重组DNA衍生的Pfs25在人类传播阻断疫苗接种研究。
Pfs25 is a sexual stage antigen of Plasmodium falciparum that is expressed on the surface of zygote and ookinete forms of the parasite. Monoclonal antibodies directed against native Pfs25 can block completely the development of P. falciparum oocysts in the midgut of the mosquito vector. Thus, this 25-kD protein is a potential vaccine candidate for eliciting transmission-blocking immunity in inhabitants of malaria endemic regions. We have synthesized, by secretion from yeast, a polypeptide analogue of Pfs25 that reacts with conformation- dependent monoclonal antibodies, and elicits transmission-blocking antibodies when used to immunize mice and monkeys in conjunction with a muramyl tripeptide adjuvant. Our results suggest the further evaluation of recombinant DNA-derived Pfs25 in transmission-blocking vaccination studies in humans.