Spatial, temporal, and hormonal regulation of epidermal keratin expression during development of the frog, Xenopus laevis.
Spatial, temporal, and hormonal regulation of epidermal keratin expression during development of the frog, Xenopus laevis.
复制标题
非洲爪蟾发育过程中表皮角蛋白表达的空间、时间和激素调节。
DOI：
10.1016/0012-1606(92)90222-3
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发表时间：
1992
影响因子：
2.7
通讯作者：
Miller,L
中科院分区：
文献类型：
--
作者：
Nishikawa,A;Shimizu-Nishikawa,K;Miller,L
为了研究激素诱导角蛋白在变态过程中在表皮中表达的机制，提出了一种针对63 kDa角蛋白羧基末端多肽的单特异性抗体。免疫组织化学分析表明，63-kDa角蛋白表达的起始时间具有明显的区域和时间差异。在后肢表皮中的表达开始于阶段54，在头部的阶段57，以及一个多月后在尾部的阶段63。63 kDa角蛋白的含量在表皮层积和分化过程中受到进一步的调节。63 kDa角蛋白在层结开始之前首先在基表皮细胞中表达。幼虫表皮外层(周皮)不表达63 kDa角蛋白。随着细胞移出基底层，它们与抗角蛋白抗体的染色更加强烈，表明63-kDa角蛋白合成在分化过程中上调。在高钙条件下生长的纯化表皮细胞的培养也得到了类似的结果。由于我们已经证明甲状腺激素(T3)诱导63 kDa角蛋白基因的表达，氢化可的松(HC)调节T3的作用，我们用抗角蛋白抗体在单细胞水平上检测了T3和HC的作用。免疫组织化学染色显示，T3组和T3+HC组的63-kDa角蛋白阳性细胞数和每细胞63-kDa角蛋白含量均增加。出乎意料的是，这些激素对头部和尾部表皮细胞的影响是相同的，尽管后者细胞在变态过程中退化。尾部细胞与头部细胞的主要区别在于，头部63 kDa角蛋白产生细胞的比例远大于尾部。
To study the mechanism of hormone-induced keratin expression in the epidermis duringXenopusmetamorphosis, a monospecific antibody was raised against a unique carboxy-terminal peptide of the 63-kDa keratin. Immunohistological analysis demonstrated that the onset of 63-kDa keratin expression showed distinct regional and temporal differences. The expression started at stage 54 in the hindlimb epidermis, at stage 57 in the head, and over 1 month later at stage 63 in the tail. The amount of 63-kDa keratin was further regulated during epidermal stratification and differentiation. The 63-kDa keratin was expressed first in basal epidermal cells before stratification began. The outer layer of the larval epidermis (periderm) did not express the 63-kDa keratin. As the cells moved out of basal layer, they stained more intensely with the anti-keratin antibody indicating that 63-kDa keratin synthesis is up-regulated during differentiation. Similar results were obtained with cultures of purified epidermal cells grown in high calcium conditions. Since we have shown that thyroid hormone (T3) induces 63-kDa keratin gene expression and hydrocortisone (HC) modulates T3action we examined the effects of T3and HC at the single cell level with the anti-keratin antibody. Immunostaining demonstrated that T3alone and T3plus HC increased the number of 63-kDa keratin-positive cells as well as the amount of 63-kDa keratin per cell. Unexpectedly these hormones had the same effects on head and tail epidermal cells even though the latter cells degenerate during metamorphosis. The major difference between tail and head cells was that the percentage 63-kDa keratin-producing cells was much greater in the head than in the tail.