Nucleotide sequence of the anticodon region of Torulopsis phenylalanine transfer RNA.
Nucleotide sequence of the anticodon region of Torulopsis phenylalanine transfer RNA.
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球拟酵母苯丙氨酸转移 RNA 反密码子区域的核苷酸序列。
DOI：
10.1093/oxfordjournals.jbchem.a130489
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
M. Goto
中科院分区：
文献类型：
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作者：
S. Takemura;H. Kasai;M. Goto
荧光核苷Y最早在酿酒酵母tRNAPhe中发现（RajBhan dary et al.(1)）。在小麦胚芽（Yw）(2)、大鼠肝脏(3)、牛肝脏(4)以及最近从兔肝脏(5)中也发现了类似的荧光物质。核苷Y和Yw位于反密码子的3•l端附近（1,2），参与苯丙氨酸密码子的识别。y碱基的结构由Nakanishi等人(6)提出，并通过合成(7)确定。他们还确定了牛肝tRNAPhe中y样碱基的结构(8)。之前的研究表明，托鲁洛普斯（Torulopsis utilis）和酿酒链球菌（S. cerevisiae）的tRNA'sPhe序列具有相似性(9)。实际上，荧光y样碱基是从T. utilis tRNAPhe部分纯化的制剂中提取的。由于该碱与y碱在纸层色谱行为和紫外吸收光谱上有所不同，我们将其命名为y碱（t= Torulopsis）。该碱基的结构已经确定（10）（见图4）。虽然y -和yw -碱基存在于an ticodon的3•l端附近的位置，但y -碱基的位置迄今为止尚不清楚。
A fluorescent nucleoside, Y, was first found in Saccharomyces cerevisiae tRNAPhe**(RajBhan dary et al.(1)). Similar fluorescent materials were also found in tRNA'sPhe from wheat germ (Yw)(2), rat liver (3), bovine liver (4), and recently from rabbit liver (5). The nucleosides Y and Yw are located adjacent to the 3• L-end-of the anticodon(1, 2), and are involved in the recognition of the phenylalanine codons. The structure of the Y-base was proposed by Nakanishi, et al.(6) and established by synthesis (7). They also determined the structure of the Y-like base in bovine liver tRNAPhe(8). Sequence similarity between tRNA'sPhe from Torulopsis utilis and S. cerevisiae was shown previously(9). Actually, fluorescent Y-like base has been extracted from a partially purified preparation of T. utilis tRNAPhe. As this base is somewhat different from Y-base as regards paper chromatographic behavior and ultraviolet absorption spectra, we have named it Yt-base(t= Torulopsis). The structure of this base has been determined(10)(see Fig. 4). While the Y-and Yw-bases are present at the position next to the 3• L-end of the an ticodon, the location of the Yt-base is so far unknown.