Structural Analysis of Articular Cartilage Using Multiphoton Microscopy: Input for Biomechanical Modeling
Structural Analysis of Articular Cartilage Using Multiphoton Microscopy: Input for Biomechanical Modeling
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DOI：
10.1109/tmi.2011.2139222
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发表时间：
2011-09-01
影响因子：
10.6
通讯作者：
Davies, Catharina de Lange
中科院分区：
文献类型：
--
作者：
Lilledahl, Magnus B.;Pierce, David M.;Davies, Catharina de Lange
采用多光子显微镜（MPM）对鸡关节软骨的三维形态进行了量化，用于连续力学建模。为了激发这一形态学研究，我们提出了一个新的3-D关节软骨有限应变本构模型的各个方面，重点是基本的承重形态：一个不均匀的，多孔（粘）弹性固体基质，由各向异性增强，（粘）弹性分散纤维织物，被一种不可压缩的流体饱和，存在于应变依赖的孔隙中。新鲜鸡软骨样品在三个正交平面上切片，用MPM成像，特别是用二次谐波成像胶原纤维。利用基于傅里叶分析的图像分析技术，我们得到了胶原纤维织物在表层的主方向性和弥散度。在中间层，采用客观阈值技术提取细胞外胶原基质所占体积分数。结合文献中提供的信息或额外的实验测试，我们展示了如何使用这些数据推导出初始固体体积分数和达西渗透率的三维图。
The 3-D morphology of chicken articular cartilage was quantified using multiphoton microscopy (MPM) for use in continuum-mechanical modeling. To motivate this morphological study we propose aspects of a new, 3-D finite strain constitutive model for articular cartilage focusing on the essential load-bearing morphology: an inhomogeneous, poro-(visco) elastic solid matrix reinforced by an anisotropic, (visco) elastic dispersed fiber fabric which is saturated by an incompressible fluid residing in strain-dependent pores. Samples of fresh chicken cartilage were sectioned in three orthogonal planes and imaged using MPM, specifically imaging the collagen fibers using second harmonic generation. Employing image analysis techniques based on Fourier analysis, we derived the principal directionality and dispersion of the collagen fiber fabric in the superficial layer. In the middle layer, objective thresholding techniques were used to extract the volume fraction occupied by extracellular collagen matrix. In conjunction with information available in the literature, or additional experimental testing, we show how this data can be used to derive a 3-D map of the initial solid volume fraction and Darcy permeability.