The arabidopsis cyclic nucleotide interactome.
The arabidopsis cyclic nucleotide interactome.
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DOI：
10.1186/s12964-016-0133-2
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发表时间：
2016-05-11
期刊：
影响因子：
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通讯作者：
Gehring C
中科院分区：
文献类型：
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作者：
Donaldson L;Meier S;Gehring C
环核苷酸已被证明在植物的许多生理过程中发挥重要的信号作用，包括光合作用和防御。尽管如此，由于下游靶蛋白仍然未知，人们对植物中环核苷酸依赖性信号传导机制知之甚少。这主要是由于生物信息学搜索未能识别蛋白激酶和磷酸二酯酶的植物同源物，而蛋白激酶和磷酸二酯酶是动物中环核苷酸的主要靶标。使用亲和纯化技术来鉴定拟南芥中的环核苷酸结合蛋白。对鉴定出的蛋白质进行计算分析，包括序列、转录共表达和功能注释分析，以评估它们在植物环核苷酸信号传导中的潜在作用。通过实验鉴定出总共十二种环核苷酸结合蛋白，包括卡尔文循环和光呼吸途径中的关键酶。重要的是，十二种蛋白质中的八种被证明含有假定的环核苷酸结合域。此外，所鉴定的蛋白质经过一氧化氮的翻译后修饰、转录共表达和注释，以在过氧化氢信号传导和防御反应中发挥作用。其中一种蛋白质——甘草酸氧化酶 1（GLYGOLATE OXIDASE 1）——一种响应假单胞菌产生过氧化氢的光呼吸酶——的活性被证明可以通过 cGMP 和一氧化氮治疗的组合来抑制。我们认为，所鉴定的蛋白质在防御反应期间作为环核苷酸、一氧化氮和活性氧信号传导之间的串扰点共同发挥作用。本文的在线版本 (doi:10.1186/s12964-016-0133-2) 包含补充材料，可供授权用户使用。
Cyclic nucleotides have been shown to play important signaling roles in many physiological processes in plants including photosynthesis and defence. Despite this, little is known about cyclic nucleotide-dependent signaling mechanisms in plants since the downstream target proteins remain unknown. This is largely due to the fact that bioinformatics searches fail to identify plant homologs of protein kinases and phosphodiesterases that are the main targets of cyclic nucleotides in animals. An affinity purification technique was used to identify cyclic nucleotide binding proteins in Arabidopsis thaliana. The identified proteins were subjected to a computational analysis that included a sequence, transcriptional co-expression and functional annotation analysis in order to assess their potential role in plant cyclic nucleotide signaling. A total of twelve cyclic nucleotide binding proteins were identified experimentally including key enzymes in the Calvin cycle and photorespiration pathway. Importantly, eight of the twelve proteins were shown to contain putative cyclic nucleotide binding domains. Moreover, the identified proteins are post-translationally modified by nitric oxide, transcriptionally co-expressed and annotated to function in hydrogen peroxide signaling and the defence response. The activity of one of these proteins, GLYGOLATE OXIDASE 1, a photorespiratory enzyme that produces hydrogen peroxide in response to Pseudomonas, was shown to be repressed by a combination of cGMP and nitric oxide treatment. We propose that the identified proteins function together as points of cross-talk between cyclic nucleotide, nitric oxide and reactive oxygen species signaling during the defence response. The online version of this article (doi:10.1186/s12964-016-0133-2) contains supplementary material, which is available to authorized users.