Structure of the human CD97 gene: Exon shuffling has generated a new type of seven-span transmembrane molecule related to the secretin receptor superfamily
Structure of the human CD97 gene: Exon shuffling has generated a new type of seven-span transmembrane molecule related to the secretin receptor superfamily
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DOI：
10.1006/geno.1996.0092
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发表时间：
1996-02-15
期刊：
影响因子：
4.4
通讯作者：
vanLier, RAW
中科院分区：
文献类型：
--
作者：
Hamann, J;Hartmann, E;vanLier, RAW
最近的EMR 1和CD 97的cDNA克隆表明存在一组新的七跨膜（7-TM）分子，可能由19号染色体短臂上的基因簇编码。这两种分子的跨膜区域与分泌素受体（SecR）超家族同源，分泌素受体是一组对哺乳动物和昆虫肽激素具有特异性的受体。与目前已知的SecR超家族成员不同，EMR 1和CD 97具有延伸的胞外区，在N末端具有几个EGF结构域。我们在此描述了人CD 97基因的组织，该基因由18个外显子组成，扩展类似于12 kb的DNA。在跨膜区域中相同的外显子-内含子位置表明CD 97基因已经从SecR超家族的祖先基因进化而来。值得注意的是，编码300个氨基酸的外显子，CD 97通过该外显子从SecR组的其他成员延伸细胞外部分，在第1阶段中优先被内含子分开。所有三个EGF结构域都由对称的1-1类外显子编码，这表明外显子改组到来自SecR超家族的前体基因的上游区域产生了这种新型的7-TM受体。(C)出版社：Academic Press，Inc.
Recent cDNA cloning of EMR1 and CD97 suggests the existence of a new group of seven-span transmembrane (7-TM) molecules, likely encoded by a gene cluster on the short arm of chromosome 19. The membrane-spanning region of both molecules is homologous to the secretin receptor (SecR) superfamily, a group of receptors with specificity for mammalian and insect peptide hormones. Unlike members of the SecR superfamily known thus far, EMR1 and CD97 have extended extracellular regions that possess several EGF domains at the N-terminus. We herein describe the organization of the human CD97 gene, which consists of 18 exons expanding similar to 12 kb of DNA. Identical exon-intron positions in the transmembrane region indicate that the CD97 gene has evolved from an ancestral gene of the SecR superfamily. Remarkably, exons encoding the 300 amino acids by which CD97 extends the extracellular part from other members of the SecR group are preferentially separated by introns in phase 1, All three EGF domains are encoded by symmetrical class 1-1 exons, suggesting that exon shuffling to the upstream region of a precursor gene from the SecR superfamily has generated this new type of 7-TM receptor. (C) 1996 Academic Press, Inc.