Isolation and Some Properties of an Iron-Oxidizing Bacterium Thiobacillus ferrooxidans Resistant to Bisulfite Ion
Isolation and Some Properties of an Iron-Oxidizing Bacterium Thiobacillus ferrooxidans Resistant to Bisulfite Ion
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一株耐亚硫酸氢离子的铁氧化硫杆菌氧化亚铁硫杆菌的分离及部分性质
DOI：
10.1271/bbb.59.435
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
T. Tano
中科院分区：
文献类型：
--
作者：
T. Sugio;Ikuko Makino;K. Iwahori;Satomi Uemura;M. Takai;T. Tano
从自然界中分离到的75株铁氧化细菌中，只有一株氧化亚铁硫杆菌Funis 2-1能在含1.25mm钼酸钠（Mo ~（6+））的Fe ~（2+）培养基上生长。与此相反，T.铁氧化物AP 19 -3是钼敏感菌株的代表，在含1.0mM Na 2SO 4的Fe ~（2+）培养基上不能生长。通过比较Mo ~（6+）和Mo ~（5+）对铁氧化酶和细胞色素c氧化酶的抑制程度，发现Mo ~（5+）对铁氧化酶系统，尤其是细胞色素c氧化酶的抑制作用是强的，而Mo ~（6+）对铁氧化酶系统的抑制作用不是强的。Funis 2-1的细胞色素c氧化酶比AP 19 -3对Mo 5+的抗性更强。与Mo 6+相比，Mo 5+与T.氧化亚铁与AP 19 -3相比，Funis 2-1细胞显示出较低的Mo 6+或Mo 5+结合活性。T.已知氧化亚铁不仅催化还原的哺乳动物细胞色素c的氧化，而且催化Mo 5+的氧化。Mo 5 +-氧化活性测定与完整的...
Among seventy five strains of iron-oxidizing bacteria obtained from natural environments, only one strain, Thiobacillus ferrooxidans Funis 2-1, grew on Fe2+-medium with 1.25 mm of sodium molybdate (Mo6+). In contrast, T. ferrooxidans AP19-3, the representative of Mo sensitive strains, could not grow on Fe2+-medium with 1.0mm of sodium molybdate. By comparing the levels of inhibition of iron oxidase and cytochrome c oxidase by Mo6+ or Mo5+, it was found that Mo5+ but not Mo6+ is an actual inhibitor for the iron oxidation enzyme system, especially for cytochrome c oxidase. Cytochrome c oxidase of Funis 2-1 was more resistant to Mo5+ than AP19-3. Mo5+, compared to Mo6+, strongly binds to both cells and the plasma membrane of T. ferrooxidans. Funis 2-1 cells showed a lower binding activity to Mo6+ or Mo5 + compared to AP19-3. Cytochrome c oxidase of T. ferrooxidans has been known to catalyze the oxidation of not only reduced mammalian cytochrome c but also Mo5+ . Mo5+-oxidizing activities measured with intact...