ANTIOXIDANT ACTIVITY OF ALBUMIN-BOUND BILIRUBIN
ANTIOXIDANT ACTIVITY OF ALBUMIN-BOUND BILIRUBIN
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DOI：
10.1073/pnas.84.16.5918
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发表时间：
1987-08-01
影响因子：
11.1
通讯作者：
AMES, BN
中科院分区：
文献类型：
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作者：
STOCKER, R;GLAZER, AN;AMES, BN
当胆红素与人白蛋白结合并以正常人血浆中存在的浓度时，可在体外保护与白蛋白结合的亚油酸免受过氧自由基诱导的氧化。最初，白蛋白结合胆红素 (Alb-BR) 以与过氧自由基形成相同的速率被氧化，并且胆绿素作为氧化产物按化学计量产生。在等摩尔基础上，Alb-BR 成功地与尿酸竞争过氧自由基，但清除这些自由基的效率低于维生素 C。这些结果表明，1 mol Alb-BR 可以清除 2 mol 过氧自由基，并且少量的血浆胆红素足以防止白蛋白结合脂肪酸以及蛋白质本身的氧化。数据表明 Alb-BR 作为血浆和血管外空间中的生理抗氧化剂的作用。
Bilirubin, when bound to human albumin and at concentrations present in normal human plasma, protects albumin-bound linoleic acid from peroxyl radical-induced oxidation in vitro. Initially, albumin-bound bilirubin (Alb-BR) is oxidized at the same rate as peroxyl radicals are formed and biliverdin is produced stoichiometrically as the oxidation product. On an equimolar basis, Alb-BR successfully competes with uric acid for peroxyl radicals but is less efficient in scavenging these radicals than vitamin C. These results show that 1 mol of Alb-BR can scavenge 2 mol of peroxyl radicals and that small amounts of plasma bilirubin are sufficient to prevent oxidation of albumin-bound fatty acids as well as of the protein itself. The data indicate a role for Alb-BR as a physiological antioxidant in plasma and the extravascular space.