Chromosome banding studies in acute leukaemia at diagnosis.
Chromosome banding studies in acute leukaemia at diagnosis.
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急性白血病诊断时的染色体显带研究。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
R. Hardisty
中科院分区：
文献类型：
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作者：
Sylvia D. Lawler;Lorna M. Secker Walker;B. Summersgill;B. Reeves;J. Lewis;H.E.M. Kay;R. Hardisty
已尝试对 93 例诊断时的急性白血病病例进行染色体显带技术的细胞遗传学研究。由于固定中期的模糊外观，急性白血病患者的骨髓染色体中的带状图案很难可视化。急性淋巴细胞（ALL）患者染色体异常的比例高于急性髓细胞（AML）白血病。其他细胞学类型的所有病例均存在异常。超二倍体是 ALL 和 AML 中最常见的数值错误，但更大比例的 ALL 患者超过 30% 的细胞具有超二倍体。在ALL中，通常发现超二倍体的频率越高，每个细胞的染色体数量就越多。仅在 6 名患者中发现了不可归因于随机丢失的亚二倍体。在所有类型的白血病中都发现了通过重排或标记染色体鉴定的克隆。在 1 名患有 ALL 的成人和 2 名患有 AML 的儿童中鉴定出以 7q 染色体标记的克隆，其中断裂点相同。在大部分患者的骨髓染色体中发现高频率的随机干扰染色体断裂可能与疾病过程有关。
Cytogenetic study by a chromosome banding technique has been attempted in 93 cases of acute leukaemia at diagnosis. Banding patterns were difficult to visualise in the bone-marrow chromosomes of patients with acute leukaemia because of the fuzzy appearance of the fixed metaphases. The proportion of patients with abnormal chromosomes was higher in acute lymphoblastic (ALL) than in acute myeloid (AML) leukaemia. Abnormalities were present in all cases of other cytological types. Hyperdiploidy was the most commonly found numerical error in both ALL and AML but a larger proportion of patients with ALL had hyperdiploidy in more than 30% of the cells. In ALL it was generally found that the higher the frequency of hyperdiploidy the greater was the number of chromosomes per cell. Hypodiploidy not attributable to random losses was found in only 6 patients. Clones identified by rearranged or marker chromosomes were found in all types of leukaemia. Clones marked by a 7q-chromosome, in which the break point was the same, were identified in 1 adult with ALL and 2 children with AML. The high frequency of randomly disturbed chromosomal breakage found in the bone-marrow chromosomes of a high proportion of the patients may be related to the disease process.