THE IMPACT OF ADJACENT ISOTROPIC FLUIDS ON ELECTROGRAMS FROM ANISOTROPIC CARDIAC-MUSCLE - A MODELING STUDY
THE IMPACT OF ADJACENT ISOTROPIC FLUIDS ON ELECTROGRAMS FROM ANISOTROPIC CARDIAC-MUSCLE - A MODELING STUDY
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DOI：
10.1161/01.res.51.5.602
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发表时间：
1982-01-01
影响因子：
20.1
通讯作者：
MILLER, WT
中科院分区：
文献类型：
--
作者：
GESELOWITZ, DB;BARR, RC;MILLER, WT
最近的研究报告了良好的协议之间的细胞外电位记录在表面的组织浴制备和计算电位来自细胞内动作电位，假设间隙空间是无界的，均匀的和各向同性的。事实上，心肌是电各向异性的。为了研究间质各向异性的影响，开发了一种计算机模型，其中心肌和灌注液由电阻器的三维网格表示。通过认为细胞内空间和间质空间是互穿结构域或合胞体的心脏的双合胞体模型，电源与跨膜心脏动作电位相关。源受细胞内各向异性的影响。模型研究给出了以下结果：模型与其他人报道的组织浴制备和绝缘心脏上心外膜电位之间有很好的一致性;为了更好地近似，当组织浸没在灌注液中时，在计算组织表面处的细胞外电位时可以忽略间质各向异性，尽管计算值和实验值之间存在差异，但与观察到的值一致;当电极穿透组织或当流体水平下降到1 mm以下时，间隙各向异性变得重要;电位的显着影响的存在下，一个非常薄的流体层，潜在的振幅的比例因子是一致的理论模型先前推导。
Recent studies have reported good agreement between extracellular potentials recorded at the surface of a tissue bath preparation and calculated potentials derived from intracellular action potentials, assuming interstitial space is unbounded, homogeneous and isotropic. In fact, heart muscle is electrically anisotropic. To investigate the effect of interstitial anisotropy, a computer model was developed in which the heart muscle and the perfusate are represented by a 3-dimensional grid of resistors. Electric sources are related to transmembrane cardiac action potentials through a bisyncytial model of the heart which considers intracellular space and interstitial space to be interpenetrating domains or syncytia. The sources are affected by intracellular anisotropy. The model study gave the following results: there is good agreement between the model and potentials reported by others for a tissue bath preparation and for epicardial potentials on an insulated heart; to a good approximation, interstitial anisotropy can be ignored in calculating extracellular potentials at the surface of the tissue when it is immersed in perfusate, although there are differences between calculated and experimental values consistent with those observed; interstitial anisotropy becomes important when the electrode penetrates the tissue or when the fluid level drops below 1 mm; potentials are significantly affected by the presence of a very thin layer of fluid; the scale factor for potential amplitude is consistent with a theoretical model previously derived.