Constrained synaptic connectivity in functional mammalian neuronal networks grown on patterned surfaces
Constrained synaptic connectivity in functional mammalian neuronal networks grown on patterned surfaces
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DOI：
10.1016/s0165-0270(02)00077-8
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发表时间：
2002-06-30
影响因子：
3
通讯作者：
Chatenay, D
中科院分区：
文献类型：
--
作者：
Wyart, C;Ybert, C;Chatenay, D
体外有序神经元网络的使用是研究小神经元组装体发育和活动的一种有前途的方法。然而，在以前的尝试中，不能实现足够的生长控制和神经元的生理成熟。在这里，我们描述了一个原始的协议，其中聚赖氨酸模式限制细胞体的粘附规定的斑点和神经炎生长细线。这些网络中的海马神经元在体外无血清培养基中保持健康长达5周。电生理学和免疫化学显示神经元表现出成熟的兴奋性和抑制性突触，钙成像显示孤立网络中神经元的自发活动。我们证明，在这些几何网络中的神经元形成功能性突触优先于他们的第一个邻居。因此，我们已经建立了一个简单而强大的协议来约束神经元细胞体的位置和它们的连接模式。此外，网络的几何形状和生理学的长期维持提高了系统筛选药理学试剂和电子到神经元装置的新应用的可能性。(C)2002爱思唯尔科技有限公司。保留所有权利。
The use of ordered neuronal networks in vitro is a promising approach to study the development and the activity of small neuronal assemblies. However, in previous attempts, sufficient growth control and physiological maturation of neurons could not be achieved. Here we describe an original protocol in which polylysine patterns confine the adhesion of cellular bodies to prescribed spots and the neuritic growth to thin lines. Hippocampal neurons in these networks are maintained healthy in serum free medium up to 5 weeks in vitro. Electrophysiology and immunochemistry show that neurons exhibit mature excitatory and inhibitory synapses and calcium imaging reveals spontaneous activity of neurons in isolated networks. We demonstrate that neurons in these geometrical networks form functional synapses preferentially to their first neighbors. We have, therefore, established a simple and robust protocol to constrain both the location of neuronal cell bodies and their pattern of connectivity. Moreover, the long term maintenance of the geometry and the physiology of the networks raises the possibility of new applications for systematic screening of pharmacological agents and for electronic to neuron devices. (C) 2002 Elsevier Science B.V. All rights reserved.