Antiproliferative Protein from the Culture Supernatant of Lentinula Edodes C91-3 Mycelia
Antiproliferative Protein from the Culture Supernatant of Lentinula Edodes C91-3 Mycelia
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DOI：
10.1021/jf500316f
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发表时间：
2014-06-11
影响因子：
6.1
通讯作者：
Huang, Min
中科院分区：
文献类型：
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作者：
Li, Xingyun;Zhong, Mintao;Huang, Min
从香菇（Lentinula edodes）C91-3菌丝体培养上清中分离纯化了一种具有抗肿瘤活性的新蛋白LFP（91-3）A2。LFP（91-3）A2经（NH_4）（2）SO_4沉淀、离子交换层析（DEAE-cellulose）和凝胶过滤层析（Sephacryl S-200 HR）纯化。Mascot搜索结果表明，LFP（91-3）A2是一个新的蛋白质，表观分子量为26 kDa。MTT法检测LFP（91-3）A_2对肿瘤细胞增殖的抑制作用，结果表明，LFP（91-3）A_2对肿瘤细胞生长有明显抑制作用，且呈剂量依赖性（5-15 μ g/mL）（p < 0.05），对正常鸡胚成纤维细胞无毒性作用。流式细胞仪分析和透射电子显微镜观察发现LFP（91-3）A2的抗增殖机制与诱导细胞凋亡有关。LFP（91-3）A2是香菇中一种新的蛋白质，通过诱导肿瘤细胞凋亡而具有抑瘤活性，对正常细胞无毒性，有望成为临床治疗的天然产物。
We purified and isolated a novel protein (LFP(91-3)A2) with antitumor effect from Lentinula edodes C91-3 liquid mycelial culture supernatant. LFP(91-3)A2 was purified by (NH4)(2)SO4 precipitation, ion-exchange chromatography (DEAE-cellulose) and gel filtration chromatography (Sephacryl S-200HR). SDS-PAGE and MALDI-TOF/MS analysis Mascot search showed LFP(91-3)A2 is a new protein with apparent molecular weight of 26 kDa. The effect on tumor cell proliferation was assessed by using MTT assay in vitro, and the LFP(91-3)A2 reduced tumor cell growth obviously in a dose dependent manner (5-15 mu g/mL) (p < 0.05), while it exhibited no toxic effect on normal chick embryo fibroblasts. The antiproliferative mechanism of LFP(91-3)A2 was found to be associated with inducing cell apoptosis by flow cytometry analysis and transmission electron microscopy. The LFP(91-3)A2 is a novel protein from Lentinula edodes with tumor-suppressive activity via inducing apoptosis of tumor cells without toxicity on normal cells and may be beneficial to natural products in clinical treatment.