RECOMBINANT 46-KILODALTON SURFACE-ANTIGEN (P46) OF MYCOPLASMA-HYOPNEUMONIAE EXPRESSED IN ESCHERICHIA-COLI CAN BE USED FOR EARLY SPECIFIC DIAGNOSIS OF MYCOPLASMAL PNEUMONIA OF SWINE BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY
RECOMBINANT 46-KILODALTON SURFACE-ANTIGEN (P46) OF MYCOPLASMA-HYOPNEUMONIAE EXPRESSED IN ESCHERICHIA-COLI CAN BE USED FOR EARLY SPECIFIC DIAGNOSIS OF MYCOPLASMAL PNEUMONIA OF SWINE BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY
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DOI：
10.1128/jcm.33.3.680-683.1995
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发表时间：
1995-03-01
影响因子：
9.4
通讯作者：
MORI, Y
中科院分区：
文献类型：
--
作者：
FUTO, S;SETO, Y;MORI, Y
46 kDa 表面抗原 (P46) 是猪肺炎支原体的早期物种特异性免疫原性蛋白。通过体外诱变技术将P46基因中编码色氨酸的三个TGA密码子替换为TGG，利用螯合肽标签系统在大肠杆菌中表达突变的P46基因。纯化的重组 P46 成功用于酶联免疫吸附试验，检测猪血清中的猪肺炎支原体抗体。它不会与感染絮状支原体、猪鼻支原体或猪滑液支原体的猪的血清发生交叉反应。采用该方法，猪感染支原体肺炎后2周内即可检出。
The 46-kDa surface antigen (P46) is the early and species-specific immunogenic protein of Mycoplasma hyopneumoniae. Three TGA codons encoding tryptophan in the P46 gene were replaced with TGG by an in vitro mutagenesis technique, The mutated P46 gene was expressed in Escherichia coli by using the chelating peptide tag system. The purified recombinant P46 was successfully used in an enzyme-linked immunosorbent assay for detection of antibodies against M. hyopneumoniae in swine serum. It did not cross-react with sera from swine infected with Mycoplasma flocculate, Mycoplasma hyorhinis, or Mycoplasma hyosynoviae. With this method, mycoplasmal pneumonia of swine was detectable within 2 weeks after infection.