Self-assembled Nanowire Arrays as Three-dimensional Nanopores for Filtration of DNA Molecules
Self-assembled Nanowire Arrays as Three-dimensional Nanopores for Filtration of DNA Molecules
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DOI：
10.2116/analsci.31.153
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发表时间：
2015-03
影响因子：
1.6
通讯作者：
S. Rahong;T. Yasui;T. Yanagida;K. Nagashima;M. Kanai;Gang Meng;Yong He;F. Zhuge;N. Kaji;T. Kawai;Y. Baba
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文献类型：
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作者：
S. Rahong;T. Yasui;T. Yanagida;K. Nagashima;M. Kanai;Gang Meng;Yong He;F. Zhuge;N. Kaji;T. Kawai;Y. Baba
分子过滤和纯化在生物分子分析中发挥着重要作用。但仍需提高效率、减少过滤时间。在这里，我们展示了自组装纳米线阵列，作为嵌入微流体通道中的三维 (3D) 纳米孔，用于超快 DNA 过滤。 3D纳米孔结构是通过气-液-固（VLS）纳米线生长技术形成的，该技术使我们能够通过改变生长循环次数来控制过滤材料的孔径。在施加的电场下，在 1 s 内从 3D 纳米孔结构入口处的 T4 DNA (166 kbp) 混合物中过滤出 λ DNA 分子 (48.5 kbp)。此外，我们还观察了 T4 和 λ DNA 分子的单 DNA 分子迁移，以阐明过滤机制。 3D纳米孔结构具有制造简单、孔径控制的灵活性和生物分子过滤的可重复使用性。因此，它是生物分子过滤的优良材料。
Molecular filtration and purification play important roles for biomolecule analysis. However, it is still necessary to improve efficiency and reduce the filtration time. Here, we show self-assembled nanowire arrays as three-dimensional (3D) nanopores embedded in a microfluidic channel for ultrafast DNA filtration. The 3D nanopore structure was formed by a vapor-liquid-solid (VLS) nanowire growth technique, which allowed us to control pore size of the filtration material by varying the number of growth cycles. λ DNA molecules (48.5 kbp) were filtrated from a mixture of T4 DNA (166 kbp) at the entrance of the 3D nanopore structure within 1 s under an applied electric field. Moreover, we observed single DNA molecule migration of T4 and λ DNA molecules to clarify the filtration mechanism. The 3D nanopore structure has simplicity of fabrication, flexibility of pore size control and reusability for biomolecule filtration. Consequently it is an excellent material for biomolecular filtration.