Different responses of embryonic axes and cotyledons from tea seeds to desiccation and cryoexposure
Different responses of embryonic axes and cotyledons from tea seeds to desiccation and cryoexposure
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DOI：
10.1270/jsbbs1951.46.149
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发表时间：
1996
期刊：
影响因子：
2.4
通讯作者：
Y. Kuranuki;S. Yoshida
中科院分区：
文献类型：
--
作者：
Y. Kuranuki;S. Yoshida
草本植物种子是重要的遗传资源，干燥后可在液氮中进行超低温保存。然而，来自一些亚热带木本物种（如茶树）的顽拗性种子不适合冷冻保存，主要是由于高水分水平和对干燥的敏感性。当茶整个种子干燥至低于50%（干重基础）的水分水平时，生活力突然下降。为了阐明茶种子萌发的机理，我们用日本栽培品种Yabukita检测了胚轴和子叶对脱水和随后的低温暴露的敏感性。切除的胚轴是高度耐受干燥，而子叶是高度敏感的干燥，遭受严重损害时，减少水分水平低于55%。
As important genetic resources, orthodox seeds from most herbaceous plant species can be usually cryopreserved in liquid nitrogen after desiccation. Recalcitrant seeds from some subtropical woody species, such as tea plants, however, are not feasible to cryopreserve, primarily due to the high moisture level and sensitivity to desiccation. Upon desiccation of tea whole seeds to moisture level below 50 % (dry weight basis), viability declines abruptly. TO elucidate the mechanism of the recalcitrance of tea seeds, we ex-amined sensitivity of embryonic axes and cotyledons to des-iccation and to subsequent cryoexposure using a Japanese cultivar, Yabukita. The excised embryonic axes were highly tolerant to desiccation, whereas cotyledons were highly susceptible to desiccation, suffering serious damage upon reduction of moisture level below 55 %.