Isolation and Characterization of nif Mutants in Rhodopseudomonas capsulata
Isolation and Characterization of nif Mutants in Rhodopseudomonas capsulata
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荚膜红假单胞菌 nif 突变体的分离和鉴定
DOI：
10.1007/978-94-009-6923-0_383
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
A. Pühler
中科院分区：
文献类型：
--
作者：
W. Klipp;A. Pühler
利用体外构建的质粒将转座子Tn5导入到荚膜红单胞菌BIO菌株中，该质粒可被广宿主范围质粒RP 4调动，但在E.杆菌通过对含有Rh的Tn5进行复测，鉴定出固氮缺陷突变体。capsulatastreins的能力，生长在氮气作为唯一的氮源。
Transposon Tn5was introduced intoRhodopseudomonascapsulatastrain BIO by usinginvitroconstructed plasmids that are mobilizable by broad host range plasmid RP4 but do not replicate outsideE. coli. Mutants deficient in nitrogen fixation were identified by retesting Tn5containingRh. capsulatastrains for their ability to grow on N2gas as sole source of nitrogen.