Involvement of miR-21 in resistance to daunorubicin by regulating PTEN expression in the leukaemia K562 cell line
Involvement of miR-21 in resistance to daunorubicin by regulating PTEN expression in the leukaemia K562 cell line
复制标题
miR-21通过调节白血病K562细胞系中PTEN表达参与柔红霉素耐药
DOI：
10.1016/j.febslet.2010.12.027
复制
发表时间：
2011-01-21
期刊：
影响因子：
3.5
通讯作者：
Wang, Chun
中科院分区：
文献类型：
--
作者：
Bai, Haitao;Xu, Rang;Wang, Chun
最近的研究表明，microRNA-21 （miR-21）在几种类型的癌症中过表达，并有助于肿瘤对化疗的耐药。在这项研究中，我们研究了miR-21是否介导白血病细胞系K562对化疗药物柔红霉素（DNR）的耐药性。与亲本细胞系K562相比，DNR耐药细胞系K562/DNR中miR-21的表达上调。稳定转染miR-21可诱导K562耐药，而抑制K562/DNR中miR-21可增强DNR的细胞毒性。进一步的实验表明，miR-21的耐药机制涉及PI3K/Akt通路以及PTEN蛋白表达后的变化。本研究为了解白血病耐药提供了一种新的机制。(C) 2010年欧洲生化学会联合会。Elsevier b.v.版权所有。
Recent studies have shown microRNA-21 (miR-21) is overexpressed in several types of cancer and contributes to tumor resistance to chemotherapy. In this study, we investigated whether miR-21 mediated resistance of the leukaemia cell line K562 to the chemotherapeutic agent daunorubicin (DNR). miR-21 expression was upregulated in the DNR resistant cell line K562/DNR compared to its parental line K562. Stable transfection of miR-21 induced drug resistance in K562, while suppression of miR-21 in K562/DNR led to enhanced DNR cytotoxicity. Additional experiments indicate that the mechanism of miR-21 drug resistance involves the PI3K/Akt pathway and changes following PTEN protein expression. This study provides a novel mechanism for understanding leukaemia drug resistance. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.