The ionic strength dependence of the cooperativity factor for DNA melting.
The ionic strength dependence of the cooperativity factor for DNA melting.
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DNA 熔解协同因子的离子强度依赖性。
DOI：
10.1080/07391102.1987.10506380
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发表时间：
1987
影响因子：
4.4
通讯作者：
B. R. Amirikyan
中科院分区：
文献类型：
--
作者：
S. Kozyavkin;S. Mirkin;B. R. Amirikyan
位于DNA螺旋内部和末端的d（AT）24.d（AT）24段的解链温度已在宽范围的离子强度值（0.01 - 1 M Na+）中测定。协同因子是从由于其不同的边界条件而导致的拉伸的熔融温度的变化计算的。随着钠浓度从1 M降低到0.01 M，协同因子下降了三个数量级，其变化在高离子强度下比在低离子强度下更不显著。
The melting temperature for the d(AT)24.d(AT)24 stretch, located inside the DNA helix and terminally, have been determined in a wide range of ionic strength values (0.01 - 1 M Na+). The cooperativity factor was calculated from the shifts in the melting temperature of the stretch due to its different boundary conditions. With the sodium concentration decreasing from 1 M to 0.01 M the cooperativity factor dropped by three orders of magnitude, its change being less marked at high than at low ionic strength.